Hypoxia and CD11b+ Cell Influx Are Strongly Associated With Lymph Node Metastasis of Oral Cancer
Hypoxia and CD11b+ Cell Influx Are Strongly Associated With Lymph Node Metastasis of Oral Cancer
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DOI：
10.21873/anticanres.14706
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发表时间：
2020-12
影响因子：
2
通讯作者：
K. Sugiura;Shintaro Nakajima;I. Kato;M. Okubo-Sato;Yoko Nakazawa;K. Mitsudo;M. Kioi
中科院分区：
文献类型：
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作者：
K. Sugiura;Shintaro Nakajima;I. Kato;M. Okubo-Sato;Yoko Nakazawa;K. Mitsudo;M. Kioi
背景/目的：口腔癌的治疗失败主要是由于不受控制的颈淋巴结（LN）转移。我们以前报道过，CD 11b+细胞在放射后被招募到肿瘤缺氧区域，导致血管重建和复发。由于淋巴管形成与血管形成有相似之处，我们研究了手术是否会诱导缺氧并刺激淋巴管生成。材料与方法：采用原位舌癌切除小鼠模型检测CD 11b+细胞的募集和淋巴管的形成。结果：手术切除OSC-19肿瘤可诱导LN转移和缺氧，随后出现CD 11b+细胞内流。在无肿瘤或SAT肿瘤模型中未观察到这些现象。随着肿瘤的生长，在CD 11b+细胞流入区域观察到淋巴管生成的刺激。CD 11b+细胞的分布由淋巴结转移到髓窦。结论：手术诱导的口腔肿瘤缺氧可导致CD 11b+细胞浸润、淋巴管生成和淋巴结转移。
Background/Aim: Treatment failure in oral cancer is mainly caused by uncontrolled cervical lymph node (LN) metastasis. We previously reported that CD11b+ cells are recruited into tumor hypoxic areas following radiation, leading to re-vascularization and relapse. Since lymphatic vessel formation has similarities with vascular formation, we examined whether surgery induces hypoxia and stimulates lymphangiogenesis. Materials and Methods: The recruitment of CD11b+ cells and the formation of lymphatic vessels were examined using orthotopic tongue cancer mouse models with glossectomy. Results: Surgery on OSC-19 tumor induced LN metastases and hypoxia, followed by CD11b+ cell influx. These phenomena were not observed in the no tumor or SAT tumor models. Stimulation of lymphangiogenesis was observed in the CD11b+ cell influx area, as the tumor grew. The localization of CD11b+ cells was changed from the lymph nodules to the medullary sinuses. Conclusion: Surgery-induced hypoxia in oral tumors leads to CD11b+ cell infiltration, lymphangiogenesis, and LN metastasis.