NFL, THE TOBACCO HOMOLOG OF FLORICAULA AND LEAFY, IS TRANSCRIPTIONALLY EXPRESSED IN BOTH VEGETATIVE AND FLORAL MERISTEMS
NFL, THE TOBACCO HOMOLOG OF FLORICAULA AND LEAFY, IS TRANSCRIPTIONALLY EXPRESSED IN BOTH VEGETATIVE AND FLORAL MERISTEMS
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DOI：
10.1105/tpc.7.2.225
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发表时间：
1995-02-01
期刊：
影响因子：
11.6
通讯作者：
MEEKSWAGNER, DR
中科院分区：
文献类型：
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作者：
KELLY, AJ;BONNLANDER, MB;MEEKSWAGNER, DR
同源基因flicaula （Antirrhinum， FLO）和LEAFY（拟南芥，LEAFY）调控着特定的花分生组织的形成，这些单拷贝基因的转录本局限于花的分生组织和部分花器官以及Antirrhinum花的叶状苞片。基于这些观察结果，我们假设烟草中FLO和LFY同源基因的转录可能作为花承诺的分子标记。烟草是一种决定性植物，其初生茎尖在产生顶花时被消耗掉。令人惊讶的是，烟草同源物NFL1和NFL2的转录本((N)在棒材Nicoliana （F)棒材LO下双转录本/(L)棒材FY下双转录本）不仅存在于花分生组织中，而且存在于不确定的营养分生组织中。这表明FLO/LFY同源基因在顶端分生组织中的转录表达不足以启动花分生组织的发育。此外，NFL基因的转录模式鉴定了先前未描述的茎尖分生组织内的细胞亚群，这可能表明独特的功能区隔。这表明，与FLO和LFY不同，它们只在花发育过程中指定确定性，NFL基因在花和叶的祖细胞中都指定确定性。
The homologous genes FLORICAULA (FLO) of Antirrhinum and LEAFY (LFY) of Arabidopsis regulate the formation of determinate floral meristems, Transcripts of these single-copy genes are confined to floral meristems and some floral organs as well as to the leaflike bracts that subtend Antirrhinum flowers. Based on these observations, we hypothesized that the transcription of genes homologous to FLO and LFY in tobacco, a determinate plant in which the primary shoot apex is consumed in the production of a terminal flower, would serve as a molecular marker for floral commitment. Surprisingly, transcripts of the tobacco homologs NFL1 and NFL2((N) double under bar Nicoliana (F) under bar LO/(L) under bar FY) were found notonly in floral meristems, but also in indeterminate vegetative meristems. This implies that the transcriptional expression of the FLO/LFY homologous genes in the apical meristem is not sufficient for the initiation of floral meristem development. In addition, the transcript patterns of the NFL genes identified a previously undescribed subset of cells within the shoot apical meristem that may indicate unique functional compartmentalization. This suggests that, unlike FLO and LFY, which specify determinacy only during floral development, the NFL genes act to specify determinacy in the progenitor cells for both flowers and leaves.