Transcriptomics and metabolomics reveal the induction of flavonoid biosynthesis pathway in the interaction of Stylosanthes-Colletotrichum gloeosporioides
Transcriptomics and metabolomics reveal the induction of flavonoid biosynthesis pathway in the interaction of Stylosanthes-Colletotrichum gloeosporioides
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转录组学和代谢组学揭示柱花草-胶孢炭疽菌相互作用中黄酮类生物合成途径的诱导
DOI：
10.1016/j.ygeno.2021.06.004
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发表时间：
2021-06-17
期刊：
影响因子：
4.4
通讯作者：
Luo, Lijuan
中科院分区：
文献类型：
--
作者：
Jiang, Lingyan;Wu, Pengpeng;Luo, Lijuan
炭疽菌是一种寄主范围广泛的半生营养型真菌病原菌，可引起一种限制产量的病害，称为炭疽病。柱花草（Stylosanthes）是热带、亚热带地区的优势牧草，炭疽病是其主要病害之一。柱花草对炭疽病的抗性机制尚不清楚，从而阻碍了抗炭疽病品种的开发。采用时间分辨叶片转录组学、代谢组学和离体抑制试验研究柱花草对炭疽菌的防御反应。转录组学研究表明，类黄酮生物合成基因在感染过程中被显着诱导。同时，代谢组学也显示黄酮类化合物的积累增加。离体条件下，根皮素和柚皮素对炭疽菌菌丝生长有抑制作用，芹菜素、大豆苷元、槲皮素和山奈酚对炭疽菌孢子萌发有抑制作用。总之，我们的研究结果表明，柱花草科普C。通过上调类黄酮生物合成途径中的基因和化合物，为抗病育种提供潜在的靶标。
Colletotrichum, a hemibiotrophic fungal pathogen with a broad host range, causes a yield-limiting disease called anthracnose. Stylo (Stylosanthes) is a dominant pasture legume in tropics and subtropics, and anthracnose is one of its most destructive disease. Resistance mechanisms against anthracnose in stylo are poorly understood, thus hindering the development of resistant varieties. We performed time-resolved leaf transcriptomics, metabolomics and in vitro inhibition assay to investigate the defense responses against Colletotrichum gloeosporioides in stylo. Transcriptomics demonstrated that flavonoid biosynthetic genes were significantly induced during the infection. Consistently, metabolomics also showed the increased accumulation of flavonoid compounds. In vitro assays showed that phloretin and naringenin inhibited the mycelial growth, and apigenin, daidzein, quercetin and kaempferol suppressed conidial germination of Colletotrichum strains. Together, our results suggest that stylo plants cope with C. gloeosporioides by up-regulation of genes and compounds in flavonoid biosynthesis pathway, providing potential targets for resistance breeding.