Regulation of ventral midbrain patterning by Hedgehog signaling
Regulation of ventral midbrain patterning by Hedgehog signaling
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DOI：
10.1242/dev.02850
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发表时间：
2007-06-01
期刊：
影响因子：
4.6
通讯作者：
Agarwala, Seema
中科院分区：
文献类型：
--
作者：
Bayly, Roy D.;Ngo, Minhtran;Agarwala, Seema
In the developing ventral midbrain, the signaling molecule sonic hedgehog ( SHH) is sufficient to specify a striped pattern of cell fates ( midbrain arcs). Here, we asked whether and precisely how hedgehog (HH) signaling might be necessary for ventral midbrain patterning. By blocking HH signaling by in ovo misexpression of Ptc1(Delta loop2), we show that HH signaling is necessary and can act directly at a distance to specify midbrain cell fates. Ventral midbrain progenitors extinguish their dependence upon HH in a spatiotemporally complex manner, completing cell-fate specification at the periphery by Hamburger and Hamilton stage 13. Thus, patterning at the lateral periphery of the ventral midbrain is accomplished early, when the midbrain is small and the HH signal needs to travel relatively short distances ( approximately 30 cell diameters). Interestingly, single-cell injections demonstrate that patterning in the midbrain occurs within the context of cortex-like radial columns of cells that can share HH blockade and are cytoplasmically connected by gap junctions. HH blockade results in increased cell scatter, disrupting the spatial coherence of the midbrain arc pattern. Finally, HH signaling is required for the integrity and the signaling properties of the boundaries of the midbrain ( e. g. the midbrain-hindbrain boundary, the dorsoventral boundary), its perturbations resulting in abnormal cell mixing across 'leaky' borders.
