New species of Torque Teno miniviruses infecting gorillas and chimpanzees
New species of Torque Teno miniviruses infecting gorillas and chimpanzees
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DOI：
10.1016/j.virol.2015.10.016
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发表时间：
2016-01-01
期刊：
影响因子：
3.7
通讯作者：
Celer, Vladimir
中科院分区：
文献类型：
--
作者：
Hrazdilova, Kristyna;Slaninkova, Eva;Celer, Vladimir
Anelloviridae家族是由不同基因组长度、高度序列多样性、共享相同基因组组织的小的、无包膜的病毒组成。不同基因型别的感染和混合感染在人类中普遍存在。相关病毒在哺乳动物宿主中的数量已被描述，但从人类近亲--大猩猩和非人类灵长类动物--获得的数据非常有限。在这里，我们报告了用半套式聚合酶链式反应从16个圈养灵长类物种的粪便样本中确定100%的感染率。只有狮身人面像的患病率仅为8%。我们描述了大猩猩的三个新物种和黑猩猩的四个新物种的BetatorqueVirus及其在一个个体中的混合感染。这项研究也是首次报道和分析感染大猩猩的近全长TTMV基因组。我们试图对宿主粪便中的无菌病毒的完整基因组进行测序，但总是失败。更广泛地使用血液/组织材料对于了解阿内洛病毒的多样性和跨物种传播是必要的。(C)2015 Elsevier Inc.保留所有权利。
Anelloviridae family is comprised of small, non-enveloped viruses of various genome lengths, high sequence diversity, sharing the same genome organization. Infections and co-infections by different genotypes in humans are ubiquitous. Related viruses were described in number of mammalian hosts, but very limited data are available from the closest human relatives - great apes and non-human primates.Here we report the 100% prevalence determined by semi-nested PCR from fecal samples of 16 captive primate species. Only the Mandrillus sphinx, showed the prevalence only 8%. We describe three new species of gorillas' and four new species of chimpanzees' Betatorqueviruses and their co-infections in one individual. This study is also first report and analysis of nearly full length TTMV genomes infecting gorillas. Our attempts to sequence the complete genomes of anelloviruses from host feces invariably failed. Broader usage of blood /tissue material is necessary to understand the diversity and interspecies transmission of anelloviruses. (C) 2015 Elsevier Inc. All rights reserved.