Angiogenic growth factors in human dentine matrix
Angiogenic growth factors in human dentine matrix
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DOI：
10.1016/s0003-9969(00)00075-3
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发表时间：
2000-11-01
影响因子：
3
通讯作者：
Smith, AJ
中科院分区：
文献类型：
--
作者：
Roberts-Clark, DJ;Smith, AJ
生长因子在介导牙本质-牙髓复合体中细胞对损伤的反应中的重要性已得到充分认识，并且据报道，几种生长因子被隔离在牙本质基质中，在修复过程中它们可以从牙本质基质中释放。损伤部位的局部血管生成似乎对于牙髓修复的成功至关重要。这里，从人牙本质中分离出可溶性和不溶性基质组分，并通过酶联免疫吸附测定（ELISA）测量这些组分中几种重要的血管生成生长因子的含量。 EDTA 可溶性基质组分含有高浓度的血小板衍生生长因子 (PDGF-AB)、较低浓度的血管内皮生长因子 (VEGF)、胎盘生长因子 (PlGF) 和成纤维细胞生长因子 (FGF2) 以及极低浓度的表皮生长因子 (EGF)。在不溶性基质级分中未检测到FGF2或PlGF，但这些级分含有一些VEGF、较低浓度的PDGF-AB和非常低浓度的EGF。结论是牙本质基质含有血管生成生长因子，损伤后它们从基质中的释放可能对牙本质-牙髓复合体的整体修复反应做出重要贡献。 (C) 2000 Elsevier Science Ltd. 保留所有权利。
The importance of growth factors in mediating the cellular responses to injury in the dentine-pulp complex is well recognized and several growth factors are reportedly sequestered in dentine matrix from where they may be released during repair processes. Local angiogenesis at the injury site appears to be critical for successful pulpal repair. Here, soluble and insoluble matrix fractions were isolated from human dentine and the amounts of several important angiogenic growth factors in these fractions measured by enzyme-linked immunosorbent assay (ELISA). The EDTA-soluble matrix fraction contained high concentrations of platelet-derived growth factor (PDGF-AB), lower concentrations of vascular endothelial growth factor (VEGF), placenta growth factor (PlGF) and fibroblast growth factor (FGF2), and very low concentrations of epidermal growth factor (EGF). No FGF2 or PlGF could be detected in the insoluble matrix fractions, but these fractions contained some VEGF, lower concentrations of PDGF-AB and very low concentrations of EGF. It was concluded that dentine matrix contains angiogenic growth factors and that their release from the matrix after injury could make an important contribution to the overall reparative response of the dentine-pulp complex. (C) 2000 Elsevier Science Ltd. All rights reserved.