Bleomycin selectively elevates mRNA levels for procollagen and fibronectin following acute lung injury.
Bleomycin selectively elevates mRNA levels for procollagen and fibronectin following acute lung injury.
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博来霉素选择性提高急性肺损伤后原胶原和纤连蛋白的 mRNA 水平。
DOI：
10.1016/0006-291x(85)91315-4
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发表时间：
1985
影响因子：
3.1
通讯作者：
Cutroneo,KR
中科院分区：
文献类型：
--
作者：
Kelley,J;Chrin,L;Shull,S;Rowe,DW;Cutroneo,KR
我们采用放射标记cDNA探针的点印迹水化技术来研究特定mRNA含量在重塑大鼠肺细胞外基质转换控制中的作用。博来霉素滴注后，总RNA含量在肺损伤后的前5天逐渐增加一倍，在随后的9天内增加得更快。在博莱霉素注射后的第一周内，I型和III型前胶原蛋白和纤维连接蛋白的mrna选择性地富集在总RNA的2- 4倍以上。没有观察到肝脏特异性rna的类似增加，表明在肺中观察到的反应并未推广到其他器官。此外，肺中I型或III型前胶原mRNA种类的增加可能与急性损伤期间迁移到肺部的炎症细胞的涌入无关，因为肺泡灌洗获得的细胞不含前胶原mRNA。
We employed the technique of dot blot hydridization of radiolabeled cDNA probes to examine the role of specific mRNA content in the control of extracellular matrix turnover in the remodeling rat lung. Following bleomycin instillation, total RNA content gradually doubled during the first 5 days following the initial lung injury, then rose much more rapidly during the ensuing 9 days. Individual mRNAs for procollagens I and III and for fibronectin were selectively enriched 2- to 4-fold above total RNA during the first week after bleomycin instillation. No comparable increases were observed in specific RNAs from liver, indicating that the response observed in the lung was not generalized to other organs. Moreover, the increases in mRNA species for procollagen types I or III in the lung could not be related to the influx of inflammatory cells which migrate into the lungs during acute injury, as cells obtained by alveolar lavage contained no mRNAs for procollagens.