Local supply of reactive oxygen species into a tissue model by atmospheric-pressure plasma-jet exposure
Local supply of reactive oxygen species into a tissue model by atmospheric-pressure plasma-jet exposure
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DOI：
10.1063/1.5091740
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发表时间：
2019-06
影响因子：
3.2
通讯作者：
T. Kawasaki;F. Mitsugi;K. Koga;M. Shiratani
中科院分区：
文献类型：
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作者：
T. Kawasaki;F. Mitsugi;K. Koga;M. Shiratani
为了在生物医学应用中安全有效地使用等离子体技术，必须控制等离子体向组织中提供活性氧物质（ROS）。在这项研究中，ROS的二维分布后，通过等离子体射流曝光通过琼脂糖组织模型可视化使用KI-淀粉凝胶试剂，以评估当地的ROS供应。在组织模型表面的部分ROS供应诱导局部ROS供应在一个点状的形状就在等离子体暴露的斑点。含O3的气体暴露没有直接等离子体接触不能诱导局部ROS供应。因此，假设局部ROS供应由血浆特异性效应诱导。然而，结果还表明，等离子体射流与组织模型表面直接接触并不一定会引起局部ROS供应。研究了组织模型厚度对局部活性氧供应的影响，在给定的实验条件下，局部活性氧供应可穿透组织模型2 mm的深度，为了等离子体技术在生物医学中的安全有效应用，必须控制等离子体向组织中的活性氧供应。在这项研究中，ROS的二维分布后，通过等离子体射流曝光通过琼脂糖组织模型可视化使用KI-淀粉凝胶试剂，以评估当地的ROS供应。在组织模型表面的部分ROS供应诱导局部ROS供应在一个点状的形状就在等离子体暴露的斑点。含O3的气体暴露没有直接等离子体接触不能诱导局部ROS供应。因此，假设局部ROS供应由血浆特异性效应诱导。然而，结果还表明，等离子体射流与组织模型表面直接接触并不一定会引起局部ROS供应。研究了组织模型厚度对局部活性氧供应的影响，在组织模型中，局部活性氧供应可穿透2 mm的深度。
The supply of reactive oxygen species (ROSs) into a tissue by plasmas must be controlled for the safe and effective use of plasma technologies in biomedical applications. In this study, the two-dimensional distributions of ROSs after passing through an agarose tissue model by the plasma-jet exposures were visualized using a KI-starch gel reagent to evaluate the local ROS supply. Partial ROS supply on the tissue model surface induced the local ROS supply in a pointlike shape just under the plasma-exposed spot. The O3-containing gas exposure without direct plasma contact could not induce the local ROS supply. Therefore, the local ROS supply was assumed to be induced by plasma-specific effects. However, the results also indicated that the plasma jet coming in direct contact with the tissue model surface did not necessarily induce the local ROS supply. The effects of the tissue model thickness on the local ROS supply were also studied; the local ROS supply could penetrate to a depth of 2 mm in the tissue model under the given experimental conditions.The supply of reactive oxygen species (ROSs) into a tissue by plasmas must be controlled for the safe and effective use of plasma technologies in biomedical applications. In this study, the two-dimensional distributions of ROSs after passing through an agarose tissue model by the plasma-jet exposures were visualized using a KI-starch gel reagent to evaluate the local ROS supply. Partial ROS supply on the tissue model surface induced the local ROS supply in a pointlike shape just under the plasma-exposed spot. The O3-containing gas exposure without direct plasma contact could not induce the local ROS supply. Therefore, the local ROS supply was assumed to be induced by plasma-specific effects. However, the results also indicated that the plasma jet coming in direct contact with the tissue model surface did not necessarily induce the local ROS supply. The effects of the tissue model thickness on the local ROS supply were also studied; the local ROS supply could penetrate to a depth of 2 mm in the tissue mode...