Transcriptional expression of calvarial bone after treatment with low-intensity ultrasound:: An in vitro study
Transcriptional expression of calvarial bone after treatment with low-intensity ultrasound:: An in vitro study
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DOI：
10.1016/j.ultrasmedbio.2006.05.014
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发表时间：
2006-10-01
影响因子：
2.9
通讯作者：
Beziat, Jean-Luc
中科院分区：
文献类型：
--
作者：
Gleizal, Arnaud;Li, Shuli;Beziat, Jean-Luc
在成熟动物和大于2岁的人类中，颅骨缺损的再骨化能力非常小。通过低强度脉冲超声对骨损伤组织部位进行临床治疗是普遍的，但关于超声对促进修复和再生的基本过程的精确影响知之甚少。在这项研究中，我们使用实时聚合酶链反应（RT-PCR），以调查成骨相关基因的表达后，低强度超声刺激成年小鼠顶骨颅骨成骨细胞。与Runx 2途径相关的基因在刺激1、2和3天后具有显著更高的水平。因此，低强度超声似乎对体外培养的颅骨的转录基因表达有影响。（电子邮件：jean-luc. chu-lyon.fr）（c）2006年世界医学和生物学超声联合会。
The capacity to reossify a calvarial bone defect is very small in mature animals and in humans greater than 2 y of age. The clinical treatment of injured tissue sites of bones by low-intensity pulsed ultrasound is widespread, but little is known about the precise effects of ultrasound on the fundamental processes that promote repair and regeneration. In this study, we used real-time polymerase chain reaction (RT-PCR) to investigate the expression of osteogenesis-associated genes after stimulation by low-intensity ultrasound in adult mouse osteoblast from the parietal calvaria. The gene associated with the Runx2 pathway had notably higher levels after 1, 2 and 3 days of stimulation. Therefore, low-intensity ultrasound seems to have an effect on the transcriptional gene expression of the calvarial bone in vitro. (E-mail: jean-luc.beziat@chu-lyon.fr) (c) 2006 World Federation for Ultrasound in Medicine & Biology.