GENES ENCODING GLYCINE-RICH ARABIDOPSIS-THALIANA PROTEINS WITH RNA-BINDING MOTIFS ARE INFLUENCED BY COLD TREATMENT AND AN ENDOGENOUS CIRCADIAN-RHYTHM
GENES ENCODING GLYCINE-RICH ARABIDOPSIS-THALIANA PROTEINS WITH RNA-BINDING MOTIFS ARE INFLUENCED BY COLD TREATMENT AND AN ENDOGENOUS CIRCADIAN-RHYTHM
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DOI：
10.1104/pp.104.3.1015
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发表时间：
1994-03-01
期刊：
影响因子：
7.4
通讯作者：
SIMON, AE
中科院分区：
文献类型：
--
作者：
CARPENTER, CD;KREPS, JA;SIMON, AE
我们已经鉴定了一类富含甘氨酸的拟南芥RNA结合蛋白的两个成员的表达，我们将其命名为CCR1和CCR2。Southern杂交分析表明，CCR1和CCR2是一个小基因家族的成员。CCR1和CCR2的mRNA水平都受到昼夜节律的影响，这种昼夜节律对植物来说有一个不寻常的阶段，下午6：00积累最大，上午10：00积累最小然而，CCR1蛋白的水平在整个周期中保持相对恒定。CCR1和CCR2基因的转录积累模式与影响玉米、高粱和胡萝卜相似基因表达的条件有很大不同。CCR1和CCR2的mRNAs水平在受伤的植物中没有变化，在冷胁迫的植物中至少增加了4倍，在脱落酸处理的植物中减少了2-3倍。在干旱条件下，CCR1转录水平下降，而CCR2转录水平上升。基于暗生长植物中存在额外的CCR转录本，我们认为CCR转录本可能受到光或暗介导的调节。
We have characterized the expression of two members of a class of Arabidopsis thaliana glycine-rich, putative RNA-binding proteins that we denote Ccr1 and Ccr2. Southern blot analysis indicates that Ccr1 and Ccr2 are members of a small gene family. Both Ccr1 and Ccr2 mRNA levels were influenced by a circadian rhythm that has an unusual phase for plants, with maximal accumulation at 6:00 PM and minimal accumulation at 10:00 AM. The level of CCR1 protein, however, remained relatively constant throughout the cycle. The transcript accumulation patterns of the Ccr1 and Ccr2 genes differed considerably from conditions that affect the expression of similar genes from maize, sorghum, and carrot. Levels of Ccr1 and Ccr2 mRNAs were unchanged in wounded plants, increased at least 4-fold in cold-stressed plants, and decreased 2- to 3-fold in abscisic acid-treated plants. Ccr1 transcript levers decreased in response to drought, whereas Ccr2 transcript levels increased under the same conditions. Based on the presence of additional Ccr transcripts in dark-grown plants, we propose that Ccr transcripts may be subjected to a light- or dark-mediated regulation.