RECEPTOR DETERMINANTS OF HUMAN AND ANIMAL INFLUENZA-VIRUS ISOLATES - DIFFERENCES IN RECEPTOR SPECIFICITY OF THE HEMAGGLUTININ-H-3 BASED ON SPECIES OF ORIGIN
RECEPTOR DETERMINANTS OF HUMAN AND ANIMAL INFLUENZA-VIRUS ISOLATES - DIFFERENCES IN RECEPTOR SPECIFICITY OF THE HEMAGGLUTININ-H-3 BASED ON SPECIES OF ORIGIN
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DOI：
10.1016/0042-6822(83)90150-2
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发表时间：
1983-01-01
期刊：
影响因子：
3.7
通讯作者：
PAULSON, JC
中科院分区：
文献类型：
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作者：
ROGERS, GN;PAULSON, JC
流感病毒与红细胞和宿主细胞的结合是通过病毒血凝素（H）与含有唾液酸（SA）的细胞表面受体的相互作用介导的。使用酶促修饰的人红细胞检测了这种相互作用对19种人和动物流感分离物的特异性，所述人红细胞含有具有序列SA α 2，6 Gal β 1，4GlcNAc; SA α 2，3Gal β 1，4（3）GlcNAc; SA α 2，3Gal β 1，3GalNAc或SA α 2，6 GalNAc的细胞表面唾液酸寡糖。尽管没有一种病毒凝集含有SA α 2，6 GalNAc连接的细胞，但含有其它3个序列的细胞的差异凝集显示至少3种不同的受体结合类型。几种病毒分离物显示出明显的受体特异性，仅与含有SA α 2，6 Gal或SA α 2，3Gal连接的细胞结合，而其它病毒分离物与含有任一连接的细胞同样良好地结合。当存在于SA α 2，3Gal β 1，4（3）GlcNAc序列或仅含有前者的SA α 2，3Gal β 1，3GalNAc序列结合细胞中时，一些病毒可以区分含有末端SA α 2，3Gal键的2个寡糖受体决定簇。所观察到的受体特异性不受病毒神经氨酸酶的显著影响，如通过使用有效的神经氨酸酶抑制剂2-脱氧-2，3-脱氢-N-乙酰神经氨酸所示。受体特异性似乎在某种程度上取决于病毒分离的物种。特别地，H3血清型的人分离物都凝集含有SA α 2，6 Gal连接的细胞，但不凝集带有SA α 2，3Gal β 1，GalNAc序列的细胞。来自禽类和马物种的抗原相似（H3）分离物优先结合含有SA α 2，3Gal键的红细胞。鉴于禽流感病毒H3血凝素被鉴定为目前人类香港病毒上存在的H3血凝素的祖先，这一点特别令人感兴趣。
The binding of influenza virus to erythrocytes and host cells is mediated by the interaction of the viral hemagglutinin (H) with cell surface receptors containing sialic acid (SA). The specificity of this interaction for 19 human and animal influenza isolates was examined using human erythrocytes enzymatically modified to contain cell surface sialyloligosaccharides with the sequence SA.alpha.2,6Gal.beta.1,4GlcNAc; SA.alpha.2,3Gal.beta.1,4(3)GlcNAc; SA.alpha.2,3Gal.beta.1,3GalNAc or SA.alpha.2,6GalNAc. Although none of the viruses agglutinated cells containing the SA.alpha.2,6GalNAc linkage, differential agglutination of cells containing the other 3 sequences revealed at least 3 distinct receptor binding types. Several virus isolates exhibited marked receptor specificity, binding only to cells containing the SA.alpha.2,6Gal or the SA.alpha.2,3Gal linkage, while others bound equally well to cells containing either linkage. Some viruses could distinguish between 2 oligosaccharide receptor determinants containing the terminal SA.alpha.2,3Gal linkage when present in the SA.alpha.2,3Gal.beta.1,4(3)GlcNAc sequence or the SA.alpha.2,3Gal.beta.1,3GalNAc sequence binding cells containing only the former. The observed receptor specificities were not significantly influenced by the viral neuraminidases as shown by the use of the potent neuraminidase inhibitor 2-deoxy-2,3-dehydro-N-acetylneuraminic acid. Receptor specificity appeared, to some extent, to be dependent on the species from which the virus was isolated. In particular, human isolates of H3 serotype all agglutinated cells containing the SA.alpha.2,6Gal linkage, but not cells bearing the SA.alpha.2,3Gal.beta.1,GalNAc sequence. Antigenically similar (H3) isolates from avian and equine species preferentially bound erythrocytes containing the SA.alpha.2,3Gal linkages. This is of particular interest in view of the identification of the avian virus H3 hemagglutinin as the progenitor of the H3 hemagglutinin present on the current human Hong Kong viruses.