Molecular diversity of dinoflagellate symbionts of Cnidaria:: The psbA minicircle of Symbiodinium
Molecular diversity of dinoflagellate symbionts of Cnidaria:: The psbA minicircle of Symbiodinium
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DOI：
10.1016/j.protis.2005.12.002
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发表时间：
2006-06-01
期刊：
影响因子：
2.5
通讯作者：
Howe, Christopher J.
中科院分区：
文献类型：
--
作者：
Barbrook, Adrian C.;Visram, Shakil;Howe, Christopher J.
共生藻属的甲藻是珊瑚和其他底栖海洋动物内的重要共生体。共生藻的分子多样性主要通过核糖体 DNA 序列数据进行描述。我们测试了小环序列（在许多甲藻中形成叶绿体基因组）是否可以用作珊瑚和海葵中共生体分子多样性的标记。 psbA 的部分和全长序列是从广泛地理分布的珊瑚和海葵的环境样本中获得的。用部分 psbA 序列构建的系统发育树与分离株的已知系统发育型一致。进一步测序表明，psbA 基因存在于所有共生藻系统型的小环上。小环非编码部分的长度和 DNA 序列在共生藻系统发育型之间差异很大。在来自不同系统型的两个共生藻中，在非编码区发现了功能未知的重复序列的复杂模式。 psbA 小环非编码区的系统发育分析表明，小环序列可能是有用的叶绿体衍生标记，用于区分密切相关和远缘相关的共生藻分离株。 (c) 2006 年爱思唯尔有限公司。版权所有。
Dinoflagellate algae of the genus Symbiodinium are important symbionts within corals and other benthic marine animals. The molecular diversity of Symbiodinium, has been described mainly by use of ribosomal DNA sequence data. We tested whether minicircle sequences, which appear to form the chloroplast genome in many dinoflagellates, could be used as A marker for molecular diversity among symbionts found in corals and sea anemones. Partial and full-length sequences for psbA were obtained from environmental samples of coral and sea anemones of wide-ranging geographical distribution. Phylogenetic trees constructed with partial psbA sequences were consistent with the known phylotypes of the isolates. Further sequencing suggested that the psbA gene is present on a minicircle in all Symbiodinium phylotypes. The length and DNA sequence of the non-coding portion of the minicircles varied considerably among Symbiodinium phylotypes. In two Symbiodinium, isolates from different phylotypes an elaborate pattern of repeat sequences of unknown function was found in the non-coding region. Phylogenetic analysis of the non-coding region of the psbA minicircle indicates that minicircle sequences could be a useful chloroplast-derived marker for differentiating both closely related and distantly related Symbiodinium isolates. (c) 2006 Elsevier GmbH. All rights reserved.