Basigin-2 is the predominant basigin isoform that promotes tumor cell migration and invasion and correlates with poor prognosis in epithelial ovarian cancer.
Basigin-2 is the predominant basigin isoform that promotes tumor cell migration and invasion and correlates with poor prognosis in epithelial ovarian cancer.
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Basigin-2 是主要的 Basigin 亚型，可促进肿瘤细胞迁移和侵袭，并与上皮性卵巢癌的不良预后相关
DOI：
10.1186/1479-5876-11-92
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发表时间：
2013-04-08
影响因子：
7.4
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Zhao SH;Wang Y;Wen L;Zhai ZB;Ai ZH;Yao NL;Wang L;Liu WC;Chen BL;Li Y;Yang H
Basigin有四种亚型，已被证明参与各种人类癌症的进展。本研究的目的是探讨basigin-2蛋白表达在上皮性卵巢癌中的预后价值。此外，在细胞培养模型中进一步研究了basigin-2在卵巢癌中的功能。采用免疫组化方法检测146例卵巢组织中basigin-2的表达。采用Kaplan Meier分析和考克斯比例风险模型评估basigin-2与无进展生存期（PFS）和总生存期（OS）的关系。采用Real-time PCR、RT-PCR和western blot方法检测basigin-2、basigin-3和basigin-4在卵巢癌细胞系和组织中的表达。为探讨basigin-2对MMP分泌和细胞迁移侵袭的影响，将过表达载体pcDNA3.1-basigin-2和basigin-2 siRNA分别转染HO-8910和HO-8910 PM细胞。basigin-2高表达与卵巢上皮性癌淋巴管间隙受累、淋巴结转移及预后不良有关。多变量分析表明，basigin-2阳性分别是PFS（P = 0.006）和OS（P = 0.019）的独立预后因素。过表达basigin-2可增加HO-8910细胞MMP-2/9的分泌，增加肿瘤细胞的迁移和侵袭能力，而敲低basigin-2可降低HO-8910 PM细胞活性MMP-2/9的产生、迁移和侵袭能力。basigin-2的表达可能是一个独立的预后指标，抑制basigin-2的表达可能是治疗上皮性卵巢癌的一个潜在策略，尤其是在抑制和预防癌细胞侵袭和转移方面。
Basigin, which has four isoforms, has been demonstrated to be involved in progression of various human cancers. The aim of this study was to examine the prognostic value of basigin-2 protein expression in epithelial ovarian cancer. Furthermore, the function of basigin-2 in ovarian cancer was further investigated in cell culture models. Immunohistochemistry staining was performed to investigate basigin-2 expression in a total of 146 ovarian tissue specimens. Kaplan Meier analysis and Cox proportional hazards model were applied to assess the relationship between basigin-2 and progression-free survival (PFS) and overall survival (OS). Real-time PCR, RT-PCR and western blot were used to explore basigin-2, basigin-3 and basigin-4 expression in ovarian cancer cell lines and tissues. To evaluate possible contributions of basigin-2 to MMP secretion and cell migration and invasion, the overexpression vectors pcDNA3.1-basigin-2 and basigin-2 siRNA were transfected into HO-8910 and HO-8910 PM cells respectively. High basigin-2 expression was associated with lymph-vascular space involvement, lymph node metastasis and poor prognosis of epithelial ovarian cancer. Multivariate analyses indicated that basigin-2 positivity was an independent prognostic factor for PFS (P = 0.006) and OS (P = 0.019), respectively. Overexpression of basigin-2 increased the secretion of MMP-2/9 and cancer cell migration and invasion of HO-8910 cells, whereas knockdown of basigin-2 reduced active MMP-2/9 production, migration and invasion of HO-8910 PM cells. The expression of basigin-2 might be an independent prognostic marker and basigin-2 inhibition would be a potential strategy for epithelial ovarian cancer patients, especially in inhibiting and preventing cancer cell invasion and metastasis.
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DOI：
10.1007/978-1-4419-1278-7_10
发表时间：
2010-01-01
期刊：
INFECTIOUS DISEASE INFORMATICS: SYNDROMIC SURVEILLANCE FOR PUBLIC HEALTH AND BIO-DEFENSE
影响因子：
--
作者：
Chen, Hsinchun;Zeng, Daniel;Yan, Ping
通讯作者：
Yan, Ping
影响因子：
4
作者：
Davidson, B;Goldberg, I;Reich, R
通讯作者：
Reich, R
影响因子：
6.2
作者：
Buergy, Daniel;Fuchs, Tina;Allgayer, Heike
通讯作者：
Allgayer, Heike
影响因子：
4.8
作者：
Belton, Robert J., Jr.;Chen, Li;Nowak, Romana A.
通讯作者：
Nowak, Romana A.
Basigin 亚型的表征及 Basigin-3 对人肝细胞癌增殖和侵袭的抑制作用
影响因子：
5.3
作者：
Liao, Cheng-Gong;Kong, Ling-Min;Chen, Zhi-Nan
通讯作者：
Chen, Zhi-Nan