ALTERATIONS IN THE DISTRIBUTION AND ACTIVITY OF TRANSGLUTAMINASE DURING TUMOR-GROWTH AND METASTASIS
ALTERATIONS IN THE DISTRIBUTION AND ACTIVITY OF TRANSGLUTAMINASE DURING TUMOR-GROWTH AND METASTASIS
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DOI：
10.1093/carcin/6.3.459
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发表时间：
1985-01-01
期刊：
影响因子：
4.7
通讯作者：
GRIFFIN, M
中科院分区：
文献类型：
--
作者：
BARNES, RN;BUNGAY, PJ;GRIFFIN, M
在正常组织和肿瘤组织中检查转谷氨酰胺酶活性和亚细胞分布。大鼠肝脏的亚细胞分级显示转谷氨酰胺酶在颗粒级分（约 40%）和细胞质级分（约 60%）之间呈双峰分布。富集质膜级分的分离表明存在与膜相关的转谷氨酰胺酶活性，其与5''-核苷酸酶和Na+/K+-ATP酶的活性共同分布。用二乙基亚硝胺或6-对二甲氨基苯基偶氮苯并噻唑治疗诱发大鼠肝细胞癌，导致转谷氨酰胺酶活性降低，同时酶重新分布到细胞颗粒部分。当测定转谷氨酰胺酶活性的含量和分布时，荷瘤肝脏似乎代表肝细胞癌和对照肝脏之间的中间阶段。 4种可移植性大鼠肉瘤（P7、P8、MC3和CC5）的转谷氨酰胺酶活性与大鼠肝、肺和脾的正常组织相比显着降低。在检测到转移后，这种活性进一步降低发生在肉瘤 P7 和 P8 的原发生长中。转谷氨酰胺酶的分布和含量的这种变化可能是肿瘤组织的一个特征，并且对于监测和研究致癌过程可能具有价值。
Transglutaminase activity and subcellular distribution were examined in both normal and tumor tissue. Subcellular fractionation of rat liver demonstrated a bimodial distribution for transglutaminase between the particulate (.apprx. 40%) and cytosol (.apprx. 60%) fractions. Isolation of enriched plasma membrane fractions indicated the presence of membrane associated transglutaminase activity which co-distributed with that of 5''-nucleotidase and Na+/K+-ATPase. Induction of hepatocellular carcinomas in rats by treatment with either diethylnitrosamine or 6-p-dimethylaminophenylazobenzothiazole resulted in a reduction in transglutaminase activity which was accompanied by redistribution of the enzyme to the particulate fraction of the cell. The tumor bearing liver appeared to represent an intermediate stage between the hepatocellular carcinoma and control liver when assayed for content and distribution of transglutaminase activity. The transglutaminase activity of 4 transplantable rat sarcomas (P7, P8, MC3 and CC5) was greatly reduced when compared with the normal tissues of rat liver, lung and spleen. A further reduction in this activity occurred in the primary growths of the sarcomas P7 and P8 following detection of metastases. Such changes in the distribution and content of transglutaminase may be a feature of tumor tissue and may be of value in both monitoring and investigating the carcinogenic process.