A simple and compact smartphone-based device for the quantitative readout of colloidal gold lateral flow immunoassay strips
A simple and compact smartphone-based device for the quantitative readout of colloidal gold lateral flow immunoassay strips
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DOI：
10.1016/j.snb.2018.03.110
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发表时间：
2018-08-01
影响因子：
8.4
通讯作者：
Tang, Yong
中科院分区：
文献类型：
--
作者：
Xiao, Wei;Huang, Caihong;Tang, Yong
胶体金侧流免疫分析条（AuNPs-LFIS）已被广泛应用于床旁检测（POCT）的定性诊断工具。如果将条带阅读器并入，则其用途可扩展到定量分析。然而，它们的成本和非便携性使得商业条读取器无法用于家庭测试、社区或农村医院诊断。现场测试尤其如此。在这里，设计并3D打印了一个基于智能手机的阅读器，用于定量评估AuNPs-LFIS。该装置的基本原理是依靠智能手机的环境光传感器（SPALS）。利用该传感器来测量来自条带上的T线的透射光强度，透射光强度随T线上的AuNP浓度而变化。为了验证这种方法，我们新开发的智能手机的环境光传感器为基础的阅读器（SPALS阅读器）被用来读出AuNP-LFIS的三个分析目标：镉离子（Cd 2 +;检测限（LOD）为0.16 ng/mL），克伦特罗（CL; LOD为0.046 ng/mL），和猪流行性腹泻病毒（PEDV; LOD为0.055 μ g/mL）。结果显示与传统图像分析方法的结果具有良好的一致性，表明基于智能手机的设备适合用于AuNPs-LFIS读出。与传统的分析方法相比，所开发的金纳米粒子LFIS阅读器操作简单、成本低、携带方便，为金纳米粒子LFIS提供了现场定量分析工具，扩大了金纳米粒子LFIS的应用范围。(C)2018爱思唯尔B. V.保留所有权利。
Colloidal gold lateral flow immunoassay strips (AuNPs-LFIS) have been widely applied as qualitative diagnostic tools for point-of-care tests (POCT). If strip readers were incorporated, their use could be extended to quantitative analysis. However, their cost and non-portability render commercial strip readers unavailable for use in either home testing, community or rural hospital diagnosis. This is particularly true for on-site testing. Here, a smartphone-based reader was designed and 3D-printed for quantitatively assess AuNPs-LFIS. The basic principle of the devise was relying on a smartphone's ambient light sensor (SPALS). This sensor was harnessed to measure the transmitted light intensities originating from the T-lines on the strips, the transmitted light intensities vary with concentration of AuNP on the T-lines. To validate this approach, our newly developed smartphone's ambient light sensor-based reader (SPALS-reader) was used to readout AuNPs-LFIS of three analytical targets: cadmium ion (Cd2+; limit of detection (LOD) was 0.16 ng/mL), clenbuterol (CL; LOD was 0.046 ng/mL), and porcine epidemic diarrhea virus (PEDV; LOD was 0.055 mu g/mL). The result showed good consistency with the results of conventional image analysis approaches, indicating that the smartphone-based device is appropriate for use in AuNPs-LFIS readouts. Compared with the traditional analysis method, the developed AuNPs-LFIS reader is easier operated, lower cost and more portable, which provided an on-site quantitative analysis tool for AuNPs-LFIS and enhances the applied range of AuNPs-LFIS. (C) 2018 Elsevier B.V. All rights reserved.