NEONATAL SCREENING FOR CONGENITAL CYTOMEGALOVIRUS-INFECTION BY DETECTION OF VIRUS IN SALIVA
NEONATAL SCREENING FOR CONGENITAL CYTOMEGALOVIRUS-INFECTION BY DETECTION OF VIRUS IN SALIVA
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DOI：
10.1093/infdis/167.6.1433
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发表时间：
1993-06-01
影响因子：
6.4
通讯作者：
PASS, RF
中科院分区：
文献类型：
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作者：
BALCAREK, KB;WARREN, W;PASS, RF
唾液中巨细胞病毒（CMV）的快速检测作为先天性感染的筛查方法进行了评估。通过检测早期抗原荧光灶（DEAFF）和标准组织培养物（TC）来检查唾液样本。结果与尿液 DEAFF 的结果进行比较。 1870 名新生儿中，有 31 名（1.7%）的唾液中检测到 CMV，其中 26 名由 DEAFF 和 TC 检测，1 名仅由 DEAFF 检测，4 名仅由 TC 检测。这 31 名新生儿中有 28 名的尿 DEAFF 呈阳性。各项测试的灵敏度为唾液TC，96.8%；唾液聋哑人，87.1%；尿聋，90.3%。 825 个唾液样本传输介质的改变导致灵敏度提高：唾液 TC 和唾液 DEAFF，100%；尿聋，92.3%。筛查新生儿唾液中巨细胞病毒似乎至少与检测病毒尿一样敏感，是一种检测先天性感染的方法；与尿液相比，唾液的采集难度和费用都较低。
A rapid assay for detection of cytomegalovirus (CMV) in saliva was evaluated as a screening method for congenital infection. Samples of saliva were examined by detection of early antigen fluorescent foci (DEAFF) and standard tissue culture (TC). Results were compared with those from urine DEAFF. CMV was detected in saliva from 31 (1.7%) of 1870 newborns, 26 by DEAFF and TC, 1 by DEAFF alone, and 4 by TC alone. Urine DEAFF was positive in 28 of these 31 newborns. The sensitivities of various tests were saliva TC, 96.8%; saliva DEAFF, 87.1%; and urine DEAFF, 90.3%. A change in transport medium for 825 saliva samples resulted in improved sensitivities: saliva TC and saliva DEAFF, 100%; urine DEAFF, 92.3%. Screening saliva of newborns for CMV appears to be at least as sensitive a method for detecting congenital infection as detection of viruria; saliva can be collected with less difficulty and expense than urine.