The shape of myosin subfragment-1. A equivalent oblate ellipsoid model based on hydrodynamic properties.
The shape of myosin subfragment-1. A equivalent oblate ellipsoid model based on hydrodynamic properties.
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肌球蛋白亚片段1的形状。
DOI：
10.1021/bi00645a022
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发表时间：
1977
期刊：
影响因子：
2.9
通讯作者：
C. C. Wu
中科院分区：
文献类型：
--
作者：
J. T. Yang;C. C. Wu
根据沉降平衡，肌球蛋白亚片段-1的两个头部S-1（A1）和S-1（A2）的分子量分别为120 000和110 000。在流体动力学上，两个头部是不可区分的，特性粘度[eta]为0.064-0.065 dL/g，沉降系数s（0）20，w为5.8 S。连同旋转相关时间从文献（235纳秒），所有三个流体动力学特性可以更好地拟合一个等效的扁圆椭球的革命比长模型。椭圆体的赤道轴的宽度约为135 A（轴比约为6）。S-1（A1）和S-1（A2）分子可能具有半甜甜圈状或扁平的梨状形状，而不是细长的形状。
The molecular weights of the two heads of myosin subfragment-1, S-1(A1) and S-1(A2), based on sedimentation equilibrium are 120 000 and 110 000. Hydrodynamically, the two heads are indistinguishable, with intrinsic viscosity, [eta], of 0.064-0.065 dL/g and sedimentation coefficient, s(0)20,w, of 5.8 S. Together with the rotational correlation time taken from the literature (235 ns), all three hydrodynamic properties can be better fitted with an equivalent oblate ellipsoid of revolution than a prolate model. The width of the equatorial axis of the ellipsoid is about 135 A (the axial ratio is about 6). Probably, the S-1(A1) and S-1(A2) molecules have a half-doughnutlike or a flattened pearlike shape rather than an elongated one.