Involvement of the opioid system in the effects induced by nicotine on anxiety-like behaviour in mice
Involvement of the opioid system in the effects induced by nicotine on anxiety-like behaviour in mice
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DOI：
10.1007/s00213-005-2238-y
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发表时间：
2005-09-01
影响因子：
3.4
通讯作者：
Maldonado, R
中科院分区：
文献类型：
--
作者：
Balerio, GN;Aso, E;Maldonado, R
理论基础：最近的研究表明，内源性阿片系统参与了尼古丁诱导的几种行为反应，包括抗伤害性、奖励性和身体药物依赖。目的：本研究旨在探讨多种阿片受体在尼古丁诱导的抗焦虑和类焦虑反应中的可能作用。方法：急性皮下注射低剂量(0.05)和高剂量(0.8 mg/kg)尼古丁，在高架+迷宫中产生相反的效应，即抗焦虑和致焦虑样反应。动物只接触过一次尼古丁。观察阿片受体拮抗剂β-Funaltrexamine(5 mg/kg)、5-阿片拮抗剂纳曲多(2.5 mg/kg)和kappa阿片拮抗剂去甲托品(Nor-binaltorphimine，2.5 mg/kg)对尼古丁引起的抗焦虑和致焦虑反应的影响。结果：β-Funaltrexamine，而不是Nor-binaltorphimine或Naltrindole，可消除尼古丁诱导的抗焦虑作用，提示Mu-阿片受体参与了这一行为。回应。另一方面，Naltrindole，但不是Nor-binaltorphimine或beta-funaltrexamine，增加了尼古丁的焦虑样反应，表明Delta受体参与了这一行为效应。结论：内源性阿片系统参与尼古丁对焦虑样行为的影响，为进一步阐明这两个神经化学系统之间的相互作用提供了新的发现。
Rationale: Recent studies have revealed the participation of the endogenous opioid system in several behavioural responses induced by nicotine including antinociception, rewarding properties, and physical drug dependence. Objectives: The present study was designed to examine the possible involvement of the various opioid receptors in the anxiolytic- and anxiogenic-like responses induced by nicotine in mice. Methods: The acute administration of low (0.05) or high (0.8 mg/kg) doses of nicotine subcutaneously produced opposite effects in the elevated plus maze, i.e. anxiolytic- and anxiogenic-like responses, respectively. Animals were only exposed once to nicotine. The effects of the pretreatment with the mu-opioid receptor antagonist, beta-funaltrexamine (5 mg/kg), the 5-opioid antagonist, naltrindole (2.5 mg/kg) and the kappa-opioid antagonist, nor-binaltorphimine (2.5 mg/kg) intraperitoneally were evaluated on the anxiolytic- and anxiogenic-like responses induced by nicotine. Results: beta-funaltrexamine, but not nor-binaltorphimine or naltrindole, abolished nicotine-induced anxiolytic-like effects, suggesting an involvement of mu-opioid receptors in this behavioural. response. On the other hand, naltrindole, but not nor-binaltorphimine or beta-funaltrexamine, increased the anxiogenic-like responses of nicotine, suggesting an involvement of delta-receptors in this behavioural effect. Conclusions: These results demonstrate that the endogenous opioid system is involved in the effects induced by nicotine on anxiety-like behaviour and provide new findings to further clarify the interaction between these two neurochemical systems.