Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances
Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances
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DOI：
10.1016/j.jbbm.2006.08.008
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发表时间：
2007-04-10
影响因子：
--
通讯作者：
Suzutani, Tatsuo
中科院分区：
文献类型：
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作者：
Kaneko, Hisatoshi;Kawana, Takashi;Suzutani, Tatsuo
为了简化微生物的分子检测，我们评估了环介导等温扩增（LAMP）对培养基和一些生物物质的耐受性。与聚合酶链反应（PCR）相比，LAMP的灵敏度受临床样本各种成分的影响较小;因此，可以省略从样本中纯化DNA。(c)2006 Elsevier B. V.保留所有权利。
To simplify the molecular detection of micro-organisms, we evaluated the tolerance of loop-mediated isothermal amplification (LAMP) to a culture medium and some biological substances. The sensitivity of LAMP was less affected by the various components of the clinical samples than was polymerase chain reaction (PCR); therefore, DNA purification from samples could be omitted. (c) 2006 Elsevier B.V. All rights reserved.