TISSUE PH AS AN INDICATOR OF MESSENGER-RNA PRESERVATION IN HUMAN POSTMORTEM BRAIN
TISSUE PH AS AN INDICATOR OF MESSENGER-RNA PRESERVATION IN HUMAN POSTMORTEM BRAIN
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DOI：
10.1016/0169-328x(94)00219-5
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发表时间：
1995-02-01
期刊：
影响因子：
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通讯作者：
MARSDEN, CD
中科院分区：
文献类型：
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作者：
KINGSBURY, AE;FOSTER, OJF;MARSDEN, CD
应用原位杂交组织化学和寡核苷酸探针Northern杂交技术，对包括对照组和神经病理学病例在内的一大组人死后脑内的pH与mRNA保存的关系进行了研究。研究发现，组织pH值与三个脑区四种信使核糖核酸的表达有很强的相关性。低pH的组织被认为是由于长期的终端缺氧而导致的，含有减少或不存在的mRNA，而高pH的组织被发现含有可定量的量，病理脑样本的值与类似pH的对照材料的值相当。组织pH的测量提供了一种简单的方法来筛选死后大脑的mRNA保存，并被建议作为匹配人类神经退行性疾病病例对照研究的材料的一种手段。
The relationship between pH and mRNA preservation in post-mortem human brain was examined using in situ hybridization histochemistry and Northern hybridization with oligonucleotide probes in a large group of human subjects, including control and neuropathological cases. Tissue pH was found to correlate strongly with presentation of four mRNA species in three brain areas. Tissue with low pH, assumed to result from prolonged terminal hypoxia, contained reduced or absent mRNA, while tissue with higher pH was found to contain quantifiable amounts, the values for pathological brain samples being comparable to those for control material of similar pH. Measurement of tissue pH provides a simple means to screen post-mortem brain for mRNA preservation and is suggested as a means to match material in case-control studies of human neurodegenerative disease.