Prognostic values of matrix metalloproteinase family expression in human colorectal carcinoma
Prognostic values of matrix metalloproteinase family expression in human colorectal carcinoma
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DOI：
10.1016/j.jss.2007.02.011
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发表时间：
2008-05-01
影响因子：
2.2
通讯作者：
Kondo, Satoshi
中科院分区：
文献类型：
--
作者：
Asano, Toshimichi;Tada, Mitsuhiro;Kondo, Satoshi
背景。我们检测了基质金属蛋白酶（AMP）、金属蛋白酶组织抑制剂（TIMP）和带卡扎尔基序的逆转诱导富半胱氨酸蛋白（RECK）在结直肠癌组织中的表达模式，以评估其预后意义。材料和方法。采用实时逆转录-聚合酶链反应检测112例结直肠癌组织、20例正常黏膜组织和11例转移性肝病变组织中17个MMPs、4个TIMPs和RECK mRNA的表达。免疫组织化学证实了蛋白水平的表达。肿瘤与正常粘膜9个基因（MMP-1、-3、-7、-9、-10、-11、-12、-14、RECK）的表达差异极显著（P < 0.01）。原发癌和转移性病变中MMP-1、-10、-11和TIMP-1的表达差异极显著（P < 0.01）。MMP-12在无肝转移的原发性肿瘤中表达高于有肝转移的原发性肿瘤（P < 0.01）。高表达的MMP-15与较长的无病生存相关（广义Wilcoxon检验，P < 0.0062； Cox风险模型，P < 0.028，风险比0.099）。MMP， TIMP， RECK表达模式可能为了解细胞外基质降解（这是结直肠的特征）提供线索。癌症)及其在转移中的作用。(c) 2008爱思唯尔公司版权所有。
Background. We examined expression patterns of matrix metalloproteinase (AMP), tissue inhibitor of metalloproteinase (TIMP), and reversion-inducing cysteine-rich protein with Kazal motifs (RECK) in colorectal cancer tissues to assess their prognostic significance.Materials and methods. mRNA expressions of 17 MMPs, 4 TIMPs, and RECK were measured in 112 colorectal cancerous tissues, 20 normal mucosa tissues, and 11 metastatic liver lesions by real-time reverse-transcriptional-polymerase chain reaction. The protein level expressions were confirmed with immunohistochemistry.Results. Cancers and normal mucosa displayed highly significant differences (P < 0.01) in expression of nine genes (MMP-1, -3, -7, -9, -10, -11, -12, -14, and RECK). Primary cancers and metastatic lesions showed highly significant differences (P < 0.01) in MMP-1, -10, -11, and TIMP-1. MMP-12 expression was higher in the primary tumors that were associated without hepatic metastasis than those with metastasis (P < 0.01). High expression of MMP-15 was related to longer disease-free survival (generalized Wilcoxon test, P < 0.0062; Cox hazard model, P < 0.028, hazard ratio, 0.099).Conclusions. MMP, TIMP, RECK expression patterns may provide an insight into extracellular matrix degrading (which is characteristic of colorectal. cancers) and its role in metastasis. (c) 2008 Elsevier Inc. All rights reserved.