Effect of bee venom acupuncture on methamphetamine-induced hyperactivity, hyperthermia and Fos expression in mice
Effect of bee venom acupuncture on methamphetamine-induced hyperactivity, hyperthermia and Fos expression in mice
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DOI：
10.1016/j.brainresbull.2010.10.002
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发表时间：
2011-01-15
影响因子：
3.8
通讯作者：
Kwon, Young Bae
中科院分区：
文献类型：
--
作者：
Kim, Kee Won;Kim, Hyun Woo;Kwon, Young Bae
针灸已被用于治疗尼古丁、酒精、可卡因和吗啡等药物成瘾。本研究旨在探讨蜂毒（BV）针刺对甲基苯丙胺（METH，1 mg/kg，s.c.）对小鼠稀释BV（20111，0.01、0.1、1和10 mg/ml生理盐水，s.c.）双侧足三里穴（ST 36）或对照穴（SP 9或GB 39或尾根）给药。通过注射到ST 36中，剂量依赖性地减少了MET诱导的活动过度和体温过高，而BV注射（1 mg/ml）到对照点并没有产生这些抑制作用。METH注射后，Fos蛋白在中脑腹侧被盖区（VTA）、黑质（SN）、蓝斑（LC）、尾壳核（CPU）等脑区的表达明显增加。有趣的是，BV（1 mg/ml）注射到ST 36进一步增加了MET诱导的NA（核心和外壳），SN和LC中的Fos表达。当我们进行坐骨神经切除或BV和利多卡因联合治疗时（BV稀释在5%利多卡因溶液中），BV对注射MET的小鼠NA，SN和LC的Fos升高的促进作用被完全阻断，表明BV诱导的周围神经刺激在BV效应中起重要作用。此外，BV的作用可被α（2）-肾上腺素受体拮抗剂咪唑克生（3 mg/kg，i. p.）完全阻断，而S受体拮抗剂普萘洛尔（10 mg/kg，i. p.）总之，这些发现表明，BV针刺ST 36可能通过激活外周神经和中枢α（2）-肾上腺素能激活来调节MET诱导的活动过度、体温过高和Fos表达。(c)2010年爱思唯尔公司All rights reserved.
Acupuncture has been used to treat drug addiction by nicotine, alcohol, cocaine and morphine. This study was designed to investigate the effect of bee venom (BV) acupuncture on hyperactivity and hyperthermia induced by acute exposure to methamphetamine (METH, 1 mg/kg, s.c.) in mice. Diluted BV (20111 of 0.01, 0.1, 1 and 10 mg/ml in saline, s.c.) was administered bilaterally into the Zusanli acupoint (ST36) or control points (SP9 or GB39 or tail base). By injection into ST36 dose dependently reduced METH-induced hyperactivity and hyperthermia, while BV injection (1 mg/ml) into control points did not produce these suppressive effects. METH injection significantly increased Fos expression in several brain regions including nucleus accumbens (NA), caudate putamen (CPU), ventral tegmental area (VTA), substantia nigra (SN) and locus coeruleus (LC). Interestingly, BV (1 mg/ml) injection into ST36 further increased METH-induced Fos expression in NA (core and shell), SN and LC. When we performed sciatic denervation or combination treatment of BV and lidocaine (BV diluted in 5% lidocaine solution), the enhancement of Fos elevation by BV was completely blocked in the NA, SN and LC in METH-injected mice, indicating that BV-induced peripheral nerve stimulation played an important role in the BV effect. Furthermore, the effects of BV were completely blocked by the alpha(2)-adrenoceptor antagonist, idazoxan (3 mg/kg, i.p.), but not by S-adrenoceptor antagonist, propranolol (10 mg/kg, i.p.). Taken together, these findings suggest that BV acupuncture into ST36 may modulate METH-induced hyperactivity, hyperthermia and Fos expression through activation of the peripheral nerve and the central alpha(2)-adrenergic activation. (c) 2010 Elsevier Inc. All rights reserved.