The Biological Basis for Surface-dependent Regulation of Osteogenesis and Implant Osseointegration.
The Biological Basis for Surface-dependent Regulation of Osteogenesis and Implant Osseointegration.
复制标题
DOI：
10.5435/jaaos-d-21-00523
复制
发表时间：
2022-07-01
影响因子：
3.2
通讯作者：
Schwartz, Zvi
中科院分区：
文献类型：
--
作者：
Boyan, Barbara D.;Berger, Michael B.;Nelson, Fred R.;Donahue, Henry J.;Schwartz, Zvi
骨髓基质细胞（MSCs）受生物材料表面的化学和物理特性的调控。当生长在钛（Ti）和钛合金表面，如钛-铝-钒（Ti6Al4V），具有模拟破骨细胞吸收坑的微观、中观和纳米尺度特征时，它们经历细胞形状的快速变化，呈现出典型的分泌性成骨细胞的柱状形态。这些细胞表现出与成骨细胞表型相关的标志物，包括骨钙素和骨桥蛋白，它们分泌与成骨相关的因子，包括骨形态发生蛋白2 （BMP2）、血管内皮生长因子（VEGF）和神经营养信号蛋白。该通路涉及整合素表达从α5β1向α2β1的转变，并通过Wnt5a而不是Wnt3a信号传导。来自这些培养物的条件培养基可以刺激人内皮细胞的血管生成，以及不在仿生基质上生长的间充质干细胞的成骨细胞分化，这表明表面可以通过类似的机制促进体内成骨。使用多种动物模型的体内研究证实，与光滑表面的钛种植体相比，具有仿生表面的种植体可以改善骨整合，比较种植体表面形貌的临床表现的荟萃分析也证实了这一点。
Bone marrow stromal cells (MSCs) are regulated by the chemical and physical features of a biomaterial surface. When grown on titanium (Ti) and Ti alloy surfaces like titanium-aluminum-vanadium (Ti6Al4V) with specific topographies that mimic the micro, meso, and nanoscale features of an osteoclast resorption pit, they undergo a rapid change in cell shape to assume a columnar morphology typical of a secretory osteoblast. These cells exhibit markers associated with an osteoblast phenotype, including osteocalcin and osteopontin and they secrete factors associated with osteogenesis, including bone morphogenetic protein 2 (BMP2), vascular endothelial growth factor (VEGF), and neurotrophic semaphorins. The pathway involves a shift in integrin expression from α5β1 to α2β1 and signaling via Wnt5a rather than Wnt3a. Conditioned media from these cultures can stimulate vasculogenesis by human endothelial cells as well as osteoblastic differentiation of MSCs not grown on the biomimetic substrate, suggesting that the surface could promote osteogenesis in vivo through similar mechanisms. In vivo studies using a variety of animal models confirm that implants with biomimetic surfaces result in improved osseointegration compared to Ti implants with smooth surfaces, as do meta-analyses comparing clinical performance of implant surface topographies.