Involvement of Lypge in the formation of eye and pineal gland in zebrafish.
Involvement of Lypge in the formation of eye and pineal gland in zebrafish.
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DOI：
10.1016/j.gene.2017.11.062
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发表时间：
2018-02
期刊：
影响因子：
3.5
通讯作者：
Dongrui Ji;Su Wang;Mingyue Li;Shicui Zhang;Hongyan Li
中科院分区：
文献类型：
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作者：
Dongrui Ji;Su Wang;Mingyue Li;Shicui Zhang;Hongyan Li
Ly-6（lymphocyte antigen-6）家族蛋白参与免疫反应、细胞迁移和粘附以及神经元兴奋性的调节。然而，关于Ly-6蛋白在胚胎发生中的功能知之甚少。在此，我们鉴定了一个GPI锚定的Ly-6成员，命名为在松果体和眼中表达的Ly-6（lypge）。全膜原位杂交揭示了该基因的动态表达模式。它在松果体和视锥细胞中有显著表达，其表达受正齿同源物5（otx 5）的调控，otx 5已被证明控制许多松果体基因的表达。此外，我们还证实了lypge基因在斑马鱼胚胎中的表达是有节律的，而且，敲除lypge基因会导致斑马鱼胚胎中形成小脑袋和小眼睛。这表明Lypge在斑马鱼早期发育中参与了眼和松果体的形成。
The proteins of Ly-6 (lymphocyte antigen-6) family are involved in the regulation of immunoreaction, cell migration and adhesion, and neuronal excitability. However, little is known about the function of Ly-6 proteins in embryogenesis. Herein, we identified a GPI anchored Ly-6 member namedly6 expressed in pineal gland and eye(lypge). Dynamic expression pattern oflypgewas revealed by whole mountin situhybridization. It was strikingly expressed in the pineal gland and cone photoreceptor, and its expression was regulated byorthodenticlehomolog5 (otx5) which has been shown to control the expression of many pineal genes. In addition, we demonstrated thatlypgewas rhythmically expressed in larvae from 4 dpf on. Moreover, knockdown oflypgeresulted in small head and small eye formed in zebrafish embryos. These suggest that Lypge is involved in the formation of the eye and pineal gland in early development of zebrafish.