Synthesis of lysinonorleucine and mass spectrometric analysis of lysinonorleucine and merodesmosine in bovine ligament and eggshell membrane
Synthesis of lysinonorleucine and mass spectrometric analysis of lysinonorleucine and merodesmosine in bovine ligament and eggshell membrane
复制标题
牛韧带和蛋壳膜中赖氨酸正亮氨酸的合成及赖氨酸正亮氨酸和半锁链素的质谱分析
DOI：
10.1016/j.abb.2023.109585
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发表时间：
2023
影响因子：
3.9
通讯作者：
Usuki Toyonobu
中科院分区：
文献类型：
--
作者：
Oishi Kaho;Mori Natsuki;Anzawa Riki;Usuki Toyonobu
弹性蛋白是一种重要的细胞外基质蛋白，有助于细胞、组织和器官的弹性。虽然交联氨基酸如锁链素和异锁链素已被确定在弹性蛋白，有关结构的细节仍然不清楚。在这项研究中，弹性蛋白交联剂，赖氨酸正亮氨酸，化学合成和检测水解牛韧带和蛋壳膜样品，利用串联质谱。在相同的样品中，还使用相同的分析方法测量了弹性蛋白的另一种交联剂Merodesmosine。所得数据应有助于阐明弹性蛋白和蛋壳膜的交联结构。
Elastin is an important extracellular matrix protein that contributes to the elasticity of cells, tissues, and organs. Although crosslinking amino acids such as desmosine and isodesmosine have been identified in elastin, details regarding the structure remain unclear. In this study, an elastin crosslinker, lysinonorleucine, was chemically synthesized and detected in hydrolyzed bovine ligament and eggshell membrane samples utilizing tandem mass spectrometry. Merodesmosine, another crosslinker of elastin, was also measured in the same samples using the same analytical method. The resulting data should aid in the elucidating the crosslinking structure of elastin and eggshell membranes.