DNA image analysis study of lesions of the gallbladder and biliary system.
DNA image analysis study of lesions of the gallbladder and biliary system.
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DOI：
10.1002/lt.500020406
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发表时间：
1996-07-01
期刊：
影响因子：
--
通讯作者：
Kraemer, B B
中科院分区：
文献类型：
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作者：
Brunt, E M;Kraemer, B B
在图像分析仪（CAS-200）上对存档石蜡包埋组织的Feulgen染色5微米切片进行了22个肝内和肝外胆道系统和胆囊病变的DNA倍体分析。病例包括肝内胆管癌（n = 6）、肝外胆管癌（n = 5）、胆囊癌（n = 11）和适当的对照。除1例II期中分化胆囊腺癌外，所有恶性肿瘤均为III期和IV期腺癌。与倍性和分期没有相关性，很可能是因为在出现时肿瘤已处于晚期。当DNA倍性与肿瘤的级别进行比较，3个高分化腺癌（2个胆管癌，1个胆管癌）主要是二倍体，然而，二倍体峰也被发现在所有3个网站的中分化腺癌和低分化腺癌的肝外胆管。最后，22例病例中有15例（68%）仅显示非整倍体人群。在22例病例中的19例中观察到多个人群;这一发现可能反映了肿瘤内异质性，并与胆囊和胆道系统恶性肿瘤的晚期和侵袭性相关。
DNA ploidy analysis of 22 lesions arising from the intrahepatic and extrahepatic biliary systems and gallbladder was performed on Feulgen-stained 5-microns sections from archival paraffin-embedded tissue on an image analyzer (CAS-200). The cases included intrahepatic cholangiocarcinoma (n = 6), extrahepatic bile duct carcinoma (n = 5), carcinoma of the gallbladder (n = 11), and appropriate controls. All malignancies were stage III and IV adenocarcinomas with the exception of 1 stage II moderately differentiated gallbladder adenocarcinoma. No correlation with ploidy and stage could be made, most likely because of the advanced stage of the tumors at the time of presentation. When DNA ploidy was compared with the grade of tumor, the 3 well-differentiated adenocarcinomas (2 cholangiocarcinomas, 1 bile duct carcinoma) were predominantly diploid; however, diploid peaks were also found in moderately differentiated adenocarcinomas of all 3 sites and in a poorly differentiated adenocarcinoma of the extrahepatic bile ducts. Finally, 15 of 22 (68%) of cases showed only aneuploid populations. Multiple populations were observed in 19 of the 22 cases; this finding may reflect intratumoral heterogeneity and correlate with the advanced stage and aggressive nature of malignancies of the gallbladder and biliary system.