Cathelicidin protects against Helicobacter pylori colonization and the associated gastritis in mice
Cathelicidin protects against Helicobacter pylori colonization and the associated gastritis in mice
复制标题
DOI：
10.1038/gt.2012.92
复制
发表时间：
2012-12
期刊：
影响因子：
5.1
通讯作者：
L. Zhang;J. Yu;C. Wong;Tkw Ling;ZJ Li;KM Chan;SX Ren;J. Shen;Rly Chan;CC Lee
中科院分区：
文献类型：
--
作者：
L. Zhang;J. Yu;C. Wong;Tkw Ling;ZJ Li;KM Chan;SX Ren;J. Shen;Rly Chan;CC Lee
放线菌素是天然免疫系统的一种抗菌肽，已被证明可调节微生物生长、伤口愈合和炎症。然而，长春花碱是否在体内控制幽门螺杆菌感染仍未被探索。本研究旨在阐明内源性和外源性小鼠中草药在抗幽门螺杆菌感染和小鼠相关胃炎中的作用。结果表明，与野生型对照相比，基因消融的小鼠幽门螺杆菌定植和相关胃炎的易感性显著增加。此外，外源性痉挛的补充，通过生物工程的痉挛分泌乳酸乳球菌菌株，降低了幽门螺杆菌在胃中的密度以及相关的炎症细胞渗透和细胞因子的产生。综上所述，这些发现表明，长春花素在体内对幽门螺杆菌感染及其相关胃炎具有保护作用。我们的研究还证明了利用转化的食品级细菌运送空肠毒素的可行性，这可能在治疗幽门螺杆菌感染方面有潜在的临床应用。
Cathelicidin, an antimicrobial peptide of the innate immune system, has been shown to modulate microbial growth, wound healing and inflammation. However, whether cathelicidin controls Helicobacter pylori infection in vivo remains unexplored. This study sought to elucidate the role of endogenous and exogenous mouse cathelicidin (CRAMP) in the protection against H. pylori infection and the associated gastritis in mice. Results showed that genetic ablation of CRAMP in mice significantly increased the susceptibility of H. pylori colonization and the associated gastritis as compared with the wild-type control. Furthermore, replenishment with exogenous CRAMP, delivered via a bioengineered CRAMP-secreting strain of Lactococcus lactis, reduced H. pylori density in the stomach as well as the associated inflammatory cell infiltration and cytokine production. Collectively, these findings indicate that cathelicidin protects against H. pylori infection and its associated gastritis in vivo. Our study also demonstrates the feasibility of using the transformed food-grade bacteria to deliver cathelicidin, which may have potential clinical applications in the treatment of H. pylori infection in humans.