Postmortem behavior of rat microRNA (miRNA) as determined by comprehensive microarray analysis
Postmortem behavior of rat microRNA (miRNA) as determined by comprehensive microarray analysis
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通过综合微阵列分析确定大鼠 microRNA (miRNA) 的死后行为
DOI：
10.1016/j.fsigss.2011.08.105
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Funayama M
中科院分区：
文献类型：
--
作者：
Hashiyada M;Usui K;Hayashizaki Y;Sakai J;Funayama M
我们使用350个大鼠miRNAs探针进行了全面的微阵列分析，确定了大鼠死后存在的一些miRNAs。我们的分析显示，237个miRNAs（67.7%）低于阈值水平，105个（30%）表达至死亡后48小时。部分miRNAs在死后表达水平不稳定。在法医应用这些知识之前，需要使用定量PCR分析和动物实验进行进一步调查，例如估计死亡时间或量化压力。
We determined a number of miRNAs exist in rats after death using comprehensive microarray analysis with 350 rat miRNAs probes. Our analysis revealed that 237 miRNAs (67.7%) were under the threshold level and 105 (30%) were expressed until 48h after death. Some miRNAs showed the unstable levels of expression at postmortem. Further investigations using quantitative PCR analysis and animal experiments are required before forensic application of this knowledge, such as estimation of time of death or quantification of stress.