The myokine decorin is regulated by contraction and involved in muscle hypertrophy
The myokine decorin is regulated by contraction and involved in muscle hypertrophy
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DOI：
10.1016/j.bbrc.2014.06.123
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发表时间：
2014-07-25
影响因子：
3.1
通讯作者：
Eckardt, Kristin
中科院分区：
文献类型：
--
作者：
Kanzleiter, Timo;Rath, Michaela;Eckardt, Kristin
定期锻炼的健康促进作用是众所周知的，肌因子可能介导其中一些作用。富含亮氨酸的小蛋白聚糖核心蛋白聚糖被描述为肌因子已有一段时间。然而，其对骨骼肌的调节和影响尚未详细研究。在这项研究中，我们报告核心蛋白聚糖的差异表达和释放响应肌肉收缩使用不同的方法。核心蛋白聚糖从收缩的人肌管中释放，并且循环核心蛋白聚糖水平响应于人类的急性阻力运动而增加。此外，在长期训练后，人类和小鼠骨骼肌中的核心蛋白聚糖表达增加。由于核心蛋白聚糖直接结合肌肉生长抑制素，一种有效的肌肉生长抑制剂，我们研究了核心蛋白聚糖在骨骼肌生长调节中的潜在功能。在小鼠骨骼肌中核心蛋白聚糖的体内过表达促进了肌生成因子Mighty的表达，该因子受肌生成抑制素负调控。我们还发现Myodl和卵泡抑素响应于核心蛋白聚糖过表达而增加。此外，参与萎缩途径的肌肉特异性泛素连接酶atroginl和MuRFl通过核心蛋白聚糖过表达而减少。总之，我们的研究结果表明，核心蛋白聚糖分泌的肌管在运动中参与调节肌肉肥大，因此可能在运动相关的骨骼肌重组过程中发挥作用。(C)2014 Elsevier Inc. All rights reserved.
The health-promoting effects of regular exercise are well known, and myokines may mediate some of these effects. The small leucine-rich proteoglycan decorin has been described as a myokine for some time. However, its regulation and impact on skeletal muscle has not been investigated in detail. In this study, we report decorin to be differentially expressed and released in response to muscle contraction using different approaches. Decorin is released from contracting human myotubes, and circulating decorin levels are increased in response to acute resistance exercise in humans. Moreover, decorin expression in skeletal muscle is increased in humans and mice after chronic training. Because decorin directly binds myostatin, a potent inhibitor of muscle growth, we investigated a potential function of decorin in the regulation of skeletal muscle growth. In vivo overexpression of decorin in murine skeletal muscle promoted expression of the pro-myogenic factor Mighty, which is negatively regulated by myostatin. We also found Myodl and follistatin to be increased in response to decorin overexpression. Moreover, muscle-specific ubiquitin ligases atroginl and MuRF1, which are involved in atrophic pathways, were reduced by decorin overexpression. In summary, our findings suggest that decorin secreted from myotubes in response to exercise is involved in the regulation of muscle hypertrophy and hence could play a role in exercise-related restructuring processes of skeletal muscle. (C) 2014 Elsevier Inc. All rights reserved.