Improved method for post-embedding cytochemistry using reduced osmium and LR white resin.
Improved method for post-embedding cytochemistry using reduced osmium and LR white resin.
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使用还原锇和 LR 白色树脂改进包埋后细胞化学方法。
DOI：
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发表时间：
1994
影响因子：
3.2
通讯作者：
S. Yamashina
中科院分区：
文献类型：
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作者：
H. Tamaki;S. Yamashina
我们建立了一种改进的包埋后细胞化学方法，通过这种方法，可以在优异的细胞超微结构上进行高度特异性的细胞化学反应。该方法是将亚铁氰化钾还原的OsO 4后固定和丙烯酸基LR白色树脂包埋相结合。它允许免疫和凝集素-金细胞化学与精细的超微结构相媲美，通过常规osmicated和环氧包埋组织获得。用还原锇固定似乎有助于保存免疫反应性和膜结构。通过这种方法，分泌蛋白（淀粉酶和胰凝乳蛋白酶原），肌动蛋白丝的多克隆抗体，105 KD高尔基体相关蛋白的单克隆抗体（GF-1），和金标记的凝集素结合位点的免疫细胞化学定位可以证明。也可能是多重染色与硫胺素焦磷酸酶的酶细胞化学和免疫细胞化学的GF-1和抗淀粉酶抗体。这种多重染色使得腮腺腺泡细胞中高尔基体网络的部分表征成为可能。
We established an improved method for post-embedding cytochemistry by which highly specific cytochemical reactions on excellent cellular ultrastructures are possible. The method is a combination of post-fixation in potassium ferrocyanide-reduced OsO4 and embedment in acrylic-based LR White resin. It permits both immuno- and lectin-gold cytochemistry with fine ultrastructures comparable to those obtained by conventionally osmicated and epoxy-embedded tissues. Fixation with reduced osmium appeared to contribute to the preservation of immunoreactivity and membranous structures. By this method, the immunocytochemical localization of secretory proteins (amylase and chymotrypsinogen), actin filaments by polyclonal antibodies, 105 KD Golgi-associated protein by a monoclonal antibody (GF-1), and binding sites for gold-labeled lectin could be demonstrated. Also possible was multiple staining with enzyme cytochemistry of thiamine pyrophosphatase and immunocytochemistry of GF-1 and anti-amylase antibodies. This multiple staining made possible partial characterization of the trans-Golgi network in parotid acinar cells.
影响因子：
3.2
作者：
BERRYMAN, MA;RODEWALD, RD
通讯作者：
RODEWALD, RD
用于免疫金电子显微镜的脑组织 Lowicryl K4M 包埋。
DOI：
10.1177/33.9.2991364
发表时间：
1985
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
Valentino,KL;Crumrine,DA;Reichardt,LF
通讯作者：
Reichardt,LF