Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer.
Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer.
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DOI：
10.1021/acsnano.5b07357
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发表时间：
2016-01-26
期刊：
影响因子：
17.1
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Li Y;Xuan J;Song Y;Qi W;He B;Wang P;Qin L
床旁（POC）检测有可能实现快速、低成本和大规模的筛查。多重生物标志物组的POC检测可以促进非小细胞肺癌（NSCLC）的早期诊断，因此可以更及时地进行手术干预以挽救生命。在此，我们报告了纳米多孔玻璃（NPG）集成的体积条形图芯片（V-Chip），用于通过肉眼检测三种NSCLC生物标志物CEA，CYFRA 21-1和SCCA的POC。NPG膜中的3D纳米结构有效地增加了抗体结合位点的数量，减少了抗体和抗原之间的扩散距离，使NPG-V-Chip的检测限低，分析时间快。我们利用NPG-V-Chip对NSCLC生物标志物组进行检测，发现检测限可达到50 pg/mL（比原始V-Chip提高10倍），总检测时间可从4小时缩短至0.5小时。然后我们检测了21例常见癌症患者血清样本中的CEA，芯片结果与临床结果具有良好的相关性。我们使用该装置进一步分析了10个肺癌样本，并证实了使用常规ELISA方法获得的结果。总之，NPG-V-Chip平台具有多重、低检测限、低成本、不需要辅助设备和快速分析时间的能力，这可以使V-Chip成为在资源有限的环境中进行定量POC检测和个性化诊断的有用平台。
Point-of-care (POC) testing has the potential to enable rapid, low-cost, and large-scale screening. POC detection of a multiplexed biomarker panel can facilitate the early diagnosis of non-small cell lung cancer (NSCLC) and, thus, may allow for more timely surgical intervention for life-saving treatment. Herein, we report the nanoporous glass (NPG) integrated volumetric bar-chart chip (V-Chip) for POC detection of the three NSCLC biomarkers CEA, CYFRA 21-1, and SCCA, by the naked eye. The 3D nanostructures in the NPG membrane efficiently increase the number of binding sites for antibodies and decrease the diffusion distance between antibody and antigen, enabling the low detection limit and rapid analysis time of the NPG-V-Chip. We utilized the NPG-V-Chip to test the NSCLC biomarker panel and found that the limit of detection can reach 50 pg/mL (10-fold improvement over the original V-Chip), and the total assay time can be decreased from 4 to 0.5 h. We then detected CEA in 21 serum samples from patients with common cancers, and the on-chip results showed good correlation with the clinical results. We further assayed 10 lung cancer samples using the device and confirmed the results obtained using conventional ELISA methods. In summary, the NPG-V-Chip platform has the ability of multiplex, low detection limit, low cost, lack of need for accessory equipment, and rapid analysis time, which may render the V-Chip a useful platform for quantitative POC detection in resource-limited settings and personalized diagnostics.