Composite interval mapping reveals a major locus influencing embryonic development rate in rainbow trout (Oncorhynchus mykiss)
Composite interval mapping reveals a major locus influencing embryonic development rate in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1093/jhered/92.1.16
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发表时间：
2001-01-01
影响因子：
3.1
通讯作者：
Thorgaard, GH
中科院分区：
文献类型：
--
作者：
Robison, BD;Wheeler, PA;Thorgaard, GH
人们对控制鲑鱼胚胎发育速度的遗传学知之甚少，尽管这一性状在野生和养殖种群的生活史中发挥着重要作用。我们通过对两个雄性遗传衍生的双单倍体虹鳟鱼家族进行数量性状基因座（QTL）分析来研究胚胎发育率的遗传学，这两个家族是由具有不同胚胎发育率的两个克隆系杂交产生的。总共 170 个双单倍体个体在 222 个标记位点 [219 个扩增片段长度多态性 (AFLP) 标记、2 个微卫星和 p53] 进行了基因分型。遗传连锁分析产生了由 27 个连锁组组成的图谱，其中 21 个标记在最小 LOD 为 3.0 和最大 theta 为 0.40 时保持不连锁。其中八个连锁群与已发表的虹鳟连锁群相匹配，复合区间作图（CIM）揭示了两个影响孵化时间的 QTL 的证据，以及第三个 QTL 的暗示性证据。这些QTL 总共占孵化时间变异的24.6%。其中一个 QTL 对发育率有很大影响，尤其是在一个双单倍体家族中，它解释了 25.6% 的孵化时间变异。还鉴定了在外源喂养开始时影响胚胎长度和重量的 QTL，对胚胎长度影响最强的 QTL (lenR13) 与对孵化时间影响最强的 QTL (tthR13) 定位在同一位置，表明单个 QTL 可能对这两个性状具有多效性效应。这些结果表明，使用双单倍体杂交设计的克隆系是分析鲑鱼复杂性状遗传基础的有效方法。
Little is known about the genetics controlling the rate of embryonic development in salmonids, despite the fact that this trait plays an important role in the life history of wild and cultured stocks. We investigated the genetics of embryonic development rate by performing an analysis of quantitative trait loci (QTL) on two families of androgenetically derived doubled haploid rainbow trout produced from a hybrid of two clonal lines with divergent embryonic development rates. A total of 170 doubled haploid individuals were genotyped at 222 marker loci [219 amplified fragment length polymorphism (AFLP) markers, 2 microsatellites, and p53]. A genetic linkage analysis resulted in a map consisting of 27 linkage groups with 21 of the markers remaining unlinked at a minimum LOD of 3.0 and maximum theta of 0.40. Eight of these linkage groups were matched to published rainbow trout linkage groups, Composite interval mapping (CIM) revealed evidence for two QTL influencing time to hatch, and suggestive evidence for a third. These QTL accounted for a total of 24.6% of the variation in time to hatch. One of these QTL had a large effect on development rate, especially in one family of doubled haploids, in which it explained 25.6% of the variance in time to hatch. QTL influencing embryonic length and weight at the commencement of exogenous feeding were also identified, The QTL with the strongest effect on embryonic length (lenR13) mapped to the same position as the QTL with the strongest effect on time to hatch (tthR13), suggesting a single QTL may have a pleiotropic effect on both these traits. These results suggest that the use of clonal lines with a doubled haploid crossing design is an effective way of analyzing the genetic basis of complex traits in salmonids.