Persistence of Brugia malayi DNA in vector and non-vector mosquitoes:: Implications for xenomonitoring and transmission monitoring of lymphatic filariasis
Persistence of Brugia malayi DNA in vector and non-vector mosquitoes:: Implications for xenomonitoring and transmission monitoring of lymphatic filariasis
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DOI：
10.4269/ajtmh.2007.76.502
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发表时间：
2007-03-01
影响因子：
3.3
通讯作者：
Weil, Gary J.
中科院分区：
文献类型：
--
作者：
Fischer, Peter;Erickson, Sara M.;Weil, Gary J.
外来监测（检测蚊子体内的丝虫幼虫或其DNA）是评估丝虫病流行性的敏感标志物，也是评估消除方案的有用工具。为了研究微丝蚴（MF）和丝虫DNA在载体主管和非主管蚊子品系的命运，我们比较了在三种不同的蚊子品系中通过解剖和定量实时聚合酶链反应（PCR）检测马来丝虫寄生虫。我们认为PCR检测丝虫幼虫比解剖法敏感得多。尤其是蚊子体内的残留物。然而，在媒介蚊子和非媒介蚊子摄入mf阳性血液后两周或更长时间内，都可以检测到寄生虫DNA。因此，虽然媒介和非媒介蚊子物种的异种监测可能是间接检测人群中丝虫寄生虫的敏感方法，但蚊子中寄生虫DNA的阳性检测结果并不一定证明研究区域正在进行传播。
Xenomonitoring (detection of filarial larvae or their DNA in mosquitoes) is a sensitive marker for assessing the endemicity of filariasis and a useful tool for evaluating elimination programs. To examine the fate of microfilariae (mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of Brugia malayi parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different mosquito strains. We conclude that PCR is much more sensitive than dissection for detecting filarial larvae., especially their remnants in mosquitoes. However, parasite DNA can be detected in both vector and non-vector mosquitoes for two weeks or longer after they ingest mf-positive blood. Thus, although xenomonitoring with vector and non-vector mosquito species may be a sensitive method for indirectly detecting filarial parasites in human populations, positive test results for parasite DNA in mosquitoes do not necessarily prove that transmission is ongoing in the study area.