Monitoring the intracellular metabolism of nucleoside phosphoramidate pronucleotides by 31P NMR.
Monitoring the intracellular metabolism of nucleoside phosphoramidate pronucleotides by 31P NMR.
复制标题
通过 31P NMR 监测核苷氨基磷酸酯前核苷酸的细胞内代谢。
DOI：
10.1081/ncn-120028341
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Wagner,CarstonR
中科院分区：
文献类型：
--
作者：
Kim,Jisook;Drontle,DanielP;Wagner,CarstonR
The intracellular metabolism of 3′‐azido‐3′‐deoxythymidine (AZT)‐(L)‐tryptophan methyl ester phosphoramidate (L‐ATO) and AZT‐(L)‐phenylalanine methyl ester phosphoramidate (L‐APO) by the human T‐lymphoblastoid cell line CCRF‐CEM (CEM‐1.3) and peripheral blood mononuclear cell line (PBMC) was investigated with high field31P NMR spectroscopy. The AZT amino acid phosphoramidates were shown to accumulate intracellularly and to be readily converted into AZT‐MP by both tissues types. Thus, the efficient delivery of nucleoside monophosphates to cells can be facilitated by nucleoside phosphoramidate pronucleotides.
