METABOLISM OF 3-METHYLHISTIDINE IN MAN
METABOLISM OF 3-METHYLHISTIDINE IN MAN
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DOI：
10.1016/0026-0495(75)90084-0
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发表时间：
1975-01-01
影响因子：
9.8
通讯作者：
GEIGER, JW
中科院分区：
文献类型：
--
作者：
LONG, CL;HAVERBERG, LN;GEIGER, JW
使用静脉注射(14C)3-甲基组氨酸在人体中研究L-3-甲基组氨酸的代谢。单次静脉注射后长达 8 小时的过期 14CO2 分析显示没有放射性，表明该化合物在人体内没有被氧化。尿样总放射性分析表明，75% 的给药剂量在 24 小时内排出，95% 在 48 小时内排出。通过流动液体闪烁技术监测柱洗脱液的尿样离子交换色谱显示仅存在两个放射性峰。洗脱这些峰所需的时间与主要排泄成分（95.5%）（14C）3-甲基组氨酸相一致，并伴有少量（4.5%）N-乙酰基-(14C)3-甲基组氨酸。 (14C)3-甲基组氨酸的血浆消失曲线表明半衰期约为 130 分钟。 3-甲基组氨酸无法氧化，其作为原始化合物及其 N-乙酰衍生物的定量排泄与其在大鼠中的代谢命运相似，因此表明 3-甲基组氨酸排泄可以为整个动物或人类受试者的肌动蛋白和肌球蛋白周转提供可靠的测量。
The metabolism of L-3-methylhistidine was studied in man using intravenously administered (14C)3-methylhistidine. Analysis for expired14CO2for periods up to 8 hr following a single intravenous injection revealed no radioactivity, indicating that this compound is not oxidized in man.Analysis of urine samples for total radioactivity showed that 75% of the administered dose was excreted in 24 hr and 95% in 48hr. Ion-exchange chromatography of urine samples with monitoring of the column eluate by a flow liquid-scintillation technique showed the presence of only two radioactive peaks. The time taken to elute these peaks was compatible with the major excretory component (95.5%) being (14C)3-methylhistidine, accompanied by a small amount (4.5%) in the form of N-acetyl-(14C)3-methylhistidine. The plasma disappearance curves of (14C)3-methylhistidine suggested a half-life of approximately 130 min.The inability to oxidize 3-methylhistidine and its quantitative excretion as the original compound as well as its N-acetyl derivative is similar to its metabolic fate in the rat and therefore suggests that 3-methylhistidine excretion may provide a reliable measure of actin and myosin turnover in the whole animal or in human subjects.