Dynamic expression of the vertebrate-specific protein Nucks during rodent embryonic development
Dynamic expression of the vertebrate-specific protein Nucks during rodent embryonic development
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DOI：
10.1016/j.gep.2013.10.001
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发表时间：
2014-01-01
影响因子：
1.2
通讯作者：
Aleporou-Marinou, Vassiliki
中科院分区：
文献类型：
--
作者：
Drosos, Yiannis;Kouloukoussa, Mirsini;Aleporou-Marinou, Vassiliki
核酪蛋白激酶和细胞周期蛋白依赖性激酶底物1（NUCKS）是一种高度磷酸化的核蛋白，在许多类型的癌症中过表达。NUCKS的灵活性及其广泛的翻译后修饰表明它是多功能的，并且它在大多数细胞类型中的表达表明管家功能。然而，Nucks蛋白在啮齿动物发育过程中的时空表达尚未报道。因此，我们研究了在大鼠和小鼠发育过程中的Nucks mRNA和蛋白的表达，免疫组化，原位杂交，Western免疫印迹和逆转录PCR分析。我们还使用BLAST分析对表达的序列标签数据库，以确定是否NUCKS同源物在无脊椎动物生物体中表达。我们发现，Nucks的表达在胚胎发育的初始阶段增加，然后逐渐下降，直到出生在所有组织中，除了神经组织和肌肉纤维。有趣的是，Nucks的表达在E13.5的迁移神经嵴细胞和外胚层来源的组织中非常强。在分析的大多数组织中，Nucks的水平与Bax和活化的半胱天冬酶-3的水平相关，这是细胞凋亡的指示。此外，Nucks在体外神经元凋亡过程中很早就上调。表达分析表明，无脊椎动物中没有与Nucks基因同源的转录本。Nucks在增殖和静止细胞中的表达及其与Bax水平和细胞凋亡的相关性强烈表明Nucks在细胞稳态中发挥着复杂的作用。此外，在无脊椎生物体中缺乏同源性表明Nucks在脊椎动物胚胎发生中的特定作用。(C)2013爱思唯尔有限公司版权所有。
The nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS) is a highly phosphorylated nuclear protein that is overexpressed in many types of cancer. The flexibility of NUCKS and its extensive posttranslational modifications indicate that it is multifunctional, and its expression in most cell types suggests a housekeeping function. However, spatiotemporal expression of the Nucks protein during rodent development has not been reported. Thus, we investigated the expression of both the Nucks mRNA and protein during rat and mouse development by immunohistochemistry, in situ hybridization, Western immunoblotting, and reverse-transcription PCR analysis. We also used BLAST analysis against expressed sequence tag databases to determine whether a NUCKS homologue is expressed in invertebrate organisms. We found that Nucks expression increased during the initial stages of embryonic development, and then gradually decreased until birth in all tissues except the nervous tissue and muscle fibers. Interestingly, the expression of Nucks was very strong in migrating neural crest cells at E13.5 and ectoderm-derived tissues. In most tissues analyzed, the levels of Nucks correlated with the levels of Bax and activated caspase-3, which are indicative of apoptosis. Moreover, Nucks was upregulated very early during neuronal apoptosis in vitro. Expression analysis revealed that no transcript with close homology to the Nucks gene was present in invertebrates. The expression of Nucks in both proliferating and quiescent cells and its correlation with Bax levels and apoptosis strongly suggest that Nucks plays complex roles in cell homeostasis. Furthermore, the lack of homology in invertebrate organisms indicates a specific role for Nucks in vertebrate embryogenesis. (C) 2013 Elsevier B.V. All rights reserved.