[Study on relationship between estrogen receptor gene polymorphism and syndrome differentiation typing of female postmenopausal osteoporosis in Traditional Chinese medicine].
[Study on relationship between estrogen receptor gene polymorphism and syndrome differentiation typing of female postmenopausal osteoporosis in Traditional Chinese medicine].
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发表时间：
2000-12
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通讯作者：
S. An;E. Li;X. Tong
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作者：
S. An;E. Li;X. Tong
目的研究雌激素基因多态性与中国女性绝经后骨质疏松症中医辨证分型的关系。方法246例绝经后妇女，年龄44-80岁，平均65.8岁，采用分子生物学方法检测Pvu Ⅱ、Xba Ⅰ限制性片段长度多态性（RFLPs），用双能X线骨密度吸收仪分别测定腰椎（L1-4）和股骨（粗隆间、股骨颈、Ward's区）的骨密度。将受试者分为肾阴虚型、肾阳虚型和肾阴阳两虚型，观察中医证型与骨密度及雌激素受体基因多态性的关系，用Pp（Pvu Ⅱ）和Xx（Xba Ⅰ）表示RFLP，大写P和X表示限制性位点的缺失。结果PPxx基因型（n = 21）的骨密度明显低于其他基因型（n = 225），（-0.71 +/- 0.46）g/cm 2，转子间（-0.31 +/- 0.58）g/cm 2，股骨颈（-0.84 ± 0.66）g/cm 2，Ward's区（-0.96 ± 0.85）g/cm 2，该基因型中医辨证分型属肾阴阳两虚型。结论雌激素受体基因RFLPs与中医辨证分型有关。
OBJECTIVE To study the relationship between estrogen gene polymorphism and TCM Syndrome Differentiation of female postmenopausal osteoporosis in China. METHODS Two hundred and forty-six Chinese postmenopausal women, age 44-80 years, mean 65.8 years, using molecular biological method to analyze the endonuclease Pvu II, Xba I restriction fragment length polymorphisms (RFLPs), with dual X-ray bone mineral density absorption meter to determine the bone mineral densities of lumbar vertebra (L1-4) and femur (intertrochanter, femur neck, Ward's region) separately. The subjects were divided into Kidney Yin deficiency type, Kidney Yang deficiency type and both Kidney Yin-Yang deficiency type, to observe the relationship between TCM and bone density as well as estrogen receptor gene polymorphism, Pp(Pvu II) and Xx(Xba I) were used to express RFLPs, the capital P and X to express the deficit of restricting sites. RESULTS Bone mineral density of PPxx gene type (n = 21) was obviously lower than that of other gene types (n = 225), lumbar (-0.71 +/- 0.46) g/cm2, intertrochanter (-0.31 +/- 0.58) g/cm2, femur neck (-0.84 +/- 0.66) g/cm2, Ward's region (-0.96 +/- 0.85) g/cm2, the TCM Syndrome Differentiation typing of this gene type belonged to both Kidney Yin-Yang deficiency type. CONCLUSION Estrogen receptor gene RFLPs is related to TCM Syndrome Differentiation typing.