Transcriptomic analysis provides insights into the immune responses and nutrition in Ostrinia furnacalis larvae parasitized by Macrocentrus cingulum
Transcriptomic analysis provides insights into the immune responses and nutrition in Ostrinia furnacalis larvae parasitized by Macrocentrus cingulum
复制标题
转录组分析提供了对扣带大螟寄生的亚洲玉米螟幼虫的免疫反应和营养的见解
DOI：
10.1002/arch.21863
复制
发表时间：
2021-12-29
影响因子：
2.2
通讯作者：
Feng, Congjing
中科院分区：
文献类型：
--
作者：
Chen, Kangkang;Song, Jiahui;Feng, Congjing
环带长茧蜂是玉米螟幼虫的主要内寄生虫。M.扣带幼虫抑制宿主的免疫反应以存活，并摄取宿主的营养以发育，直到出现。然而，目前的研究主要集中在黄蜂对寄主免疫抑制的机理上，而忽略了黄蜂对寄主免疫反应的触发和对寄主营养状况的影响。在本研究中，我们发现，寄生O。幼虫激活快速识别反应，产生溶菌酶、抗菌肽等效应物，沿着消耗海藻糖、葡萄糖甚至脂类来防御M.扣带而过氧化物酶6和超氧化物歧化酶2（SOD 2）的表达上调，细胞免疫相关基因如含硫酯蛋白2（TEP 2）和血细胞素的信使RNA（mRNA）水平也降低，表明胡蜂寄生选择性地关闭了某些免疫反应。综合以上结果，表明寄生于该菌的O.马蜂幼虫选择性地激活免疫识别反应，上调效应基因，但抑制ROS反应和细胞免疫，投入更多的能量来激发某些免疫反应，以抵御马蜂的入侵。这项研究提供了有用的信息，进一步确定的营养和先天免疫库的关键组成部分，可能塑造主机寄生虫的共同进化动力学。
Macrocentrus cingulum is a principal endoparasite of Ostrinia furnacalis larvae. M. cingulum larvae repress host immune responses for survival and ingest host nutrients for development until emerging. However, most investigations focused on the mechanisms of how wasps repress the host immunity, the triggered immune responses and nutrient status altered by wasps in host are neglected. In this study, we found that parasitized O. furnacalis larvae activated fast recognition responses and produced some effectors such as lysozyme and antimicrobial peptides, along with more consumption of trehalose, glucose, and even lipid to defend against the invading M. cingulum. However, the expression of peroxidase 6 and superoxide dismutase 2 (SOD 2) was upregulated, and the messenger RNA (mRNA) levels of cellular immunity-related genes such as thioester-containing protein 2 (TEP 2) and hemocytin were also reduced, suggesting that some immune responses were selectively shut down by wasp parasitization. Taken together, all the results indicated that parasitized O. furnacalis larvae selectively activate the immune recognition response, and upregulate effector genes, but suppress ROS reaction and cellular immunity, and invest more energy to fuel certain immune responses to defend against the wasp invading. This study provides useful information for further identifying key components of the nutrition and innate immune repertoire which may shape host-parasitoid coevolutionary dynamics.