Phylogenetic diversity and antimicrobial activities of bryozoan-associated bacteria isolated from Mediterranean and Baltic Sea habitats
Phylogenetic diversity and antimicrobial activities of bryozoan-associated bacteria isolated from Mediterranean and Baltic Sea habitats
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DOI：
10.1016/j.syapm.2009.12.002
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发表时间：
2010-03-01
影响因子：
3.4
通讯作者：
Imhoff, Johannes F.
中科院分区：
文献类型：
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作者：
Heindl, Herwig;Wiese, Jutta;Imhoff, Johannes F.
到目前为止，只进行了一小部分关于微生物-苔藓虫关联的调查。大多数研究集中在少数苔藓虫物种上，没有一种研究涵盖伴生细菌的抗菌活性。在本研究中，对具有抗菌特性的苔藓虫相关细菌的比例和系统发育分类进行了调查。从波罗的海和地中海的几个地点收集了14种不同苔藓虫的21个标本。共分离出伴生菌340株，其中101株具有抗菌活性。虽然抗生素主要针对革兰氏阳性试验菌株，但16S rRNA基因测序显示分离株与革兰氏阴性菌(黄杆菌、阿尔法和伽玛变形杆菌)有从属关系。其中一株与革兰氏阳性放线菌有关。根据相似系数>；=99.5%，将这些序列分为27个系统型。由于同一系统型的成员来自不同的苔藓虫物种，因此没有揭示宿主特有的从属关系。然而，展示了特定部位的模式。Sphingomonas和Alteromonas属的菌株仅从地中海地区分离，而Shewanella、Marinomonas和弧菌相关菌株仅从波罗的海地区分离。虽然在两个栖息地都发现了假交替单胞菌附属菌株，但它们被划分为各自的系统型。16S rDNA相似系数<lt；98%的菌株可能代表新种，属于希瓦氏菌属、假交替单胞菌属和短杆菌属。(C)2010年爱思唯尔股份有限公司。版权所有。
To date, only a small number of investigations covering microbe-bryozoa associations have been carried out. Most of them have focused on a few bryozoan species and none have covered the antibacterial activities of associated bacteria. In the current study, the proportion and phylogenetic classification of Bryozoan-associated bacteria with antimicrobial properties were investigated. Twenty-one specimens of 14 different bryozoan species were collected from several sites in the Baltic and the Mediterranean Sea. A total of 340 associated bacteria were isolated, and 101 displayed antibiotic activities. While antibiosis was predominantly directed against Gram-positive test strains, 16S rRNA gene sequencing revealed affiliation of the isolates to Gram-negative classes (Flavobacteria, Alpha- and Gammaproteobacteria). One isolate was related to the Gram-positive Actinobacteria. The sequences were grouped into 27 phylotypes on the basis of similarity values >= 99.5%. A host-specific affiliation was not revealed as members of the same phylotype were derived from different bryozoan species. Site-specific patterns, however, were demonstrated. Strains of the genera Sphingomonas and Alteromonas were exclusively isolated from Mediterranean sites, whereas Shewanella, Marinomonas and Vibrio-related isolates were only from Baltic sites. Although Pseudoalteromonas affiliated strains were found in both habitats, they were separated into respective phylotypes. Isolates with 16S rDNA similarity values < 98%, which could possibly represent new species, belonged to the genera Shewanella, Pseudoalteromonas and Tenacibaculum. (C) 2010 Elsevier GmbH. All rights reserved.