Detection and identification of mammalian DNA from the gut of museum specimens of ticks.
Detection and identification of mammalian DNA from the gut of museum specimens of ticks.
复制标题
博物馆蜱虫标本肠道中哺乳动物 DNA 的检测和鉴定。
DOI：
10.1093/jmedent/29.6.1049
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发表时间：
1992
影响因子：
2.1
通讯作者：
OliverJr,JH
中科院分区：
文献类型：
--
作者：
Tobolewski,J;Kaliszewski,MJ;Colwell,RK;OliverJr,JH
建立了一种检测和鉴定酒精保存蜱肠道中哺乳动物DNA的方法。本文报道了从保存在70%乙醇中40年以上的蜱虫中成功扩增和测序宿主细胞色素B。所描述的方法显示了确定未知蜱宿主的希望。
A method for detection and identification of mammalian DNA in the gut of alcohol-preserved ticks has been developed. Successful amplification and sequencing of host cytochrome b is reported from ticks preserved in 70% alcohol for more than 40 yr. The method described shows promise for determination of unknown tick hosts.