Validation of in vivo fluorochrome concentrations measured using fluorescence molecular tomography -: art. no. 044019
Validation of in vivo fluorochrome concentrations measured using fluorescence molecular tomography -: art. no. 044019
复制标题
DOI：
10.1117/1.1993427
复制
发表时间：
2005-07-01
影响因子：
3.5
通讯作者：
Ntziachristos, V
中科院分区：
文献类型：
--
作者：
Graves, EE;Yessayan, D;Ntziachristos, V
荧光分子层析成像(FMT)是一种在生命系统中对荧光分子探针进行三维定量成像的手段。为了评估体内FMT的准确性，构建了半透明塑料管，其中包含已知浓度的Cy5.5荧光染料的混浊溶液，并将其植入裸鼠皮下，模拟肿瘤的存在，积累了荧光分子报告程序。对比植入前后荧光管的测量结果表明，FMT报告的均匀模体的准确性扩展到了活体情况。在注射了Cy5.5标记的Annexin V的小鼠身上，通过成像荧光管来测试FMT对背景荧光的敏感性。对于小管荧光浓度，背景荧光的存在会导致重建浓度的增加。这一现象可以通过对测量的荧光数据进行简单的减法校正来抵消。通过不同墨水浓度的成像荧光管来模拟不同的肿瘤光子吸收的影响，并且被发现是次要的。这些发现证明了荧光断层成像的体内定量准确性，并鼓励这种成像方式的进一步发展以及FMT在使用荧光报告的分子成像研究中的应用。(C)2005年光学仪器工程师学会。
Fluorescence molecular tomography (FMT) has emerged as a means of quantitatively imaging fluorescent molecular probes in three dimensions in living systems. To assess the accuracy of FMT in vivo, translucent plastic tubes containing a turbid solution with a known concentration of Cy 5.5 fluorescent dye are constructed and implanted subcutaneously in nude mice, simulating the presence of a tumor accumulating a fluorescent molecular reporter. Comparisons between measurements of fluorescent tubes made before and after implantation demonstrate that the accuracy of FMT reported for homogeneous phantoms extends to the in vivo situation. The sensitivity of FMT to background fluorescence is tested by imaging fluorescent tubes in mice injected with Cy 5.5-labeled Annexin V. For small tube fluorochrome concentrations, the presence of background fluorescence results in increases in the reconstructed concentration. This phenomenon is counteracted by applying a simple subtraction correction to the measured fluorescence data. The effects of varying tumor photon absorption are simulated by imaging fluorescent tubes with varying ink concentrations, and are found to be minor. These findings demonstrate the in vivo quantitative accuracy of fluorescence tomography, and encourage further development of this imaging modality as well as application of FMT in molecular imaging studies using fluorescent reporters. (C) 2005 Society of Photo-Optical Instrumentation Engineers.