Establishment of a Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics Method and Its Application to Lichen Profiling
Establishment of a Direct-Injection Electron Ionization-Mass Spectrometry Metabolomics Method and Its Application to Lichen Profiling
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DOI：
10.1021/acs.analchem.7b00077
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发表时间：
2017-06-20
影响因子：
7.4
通讯作者：
Matsuno, Koji
中科院分区：
文献类型：
--
作者：
Kai, Hisahiro;Kinoshita, Kaoru;Matsuno, Koji
直接注射电子电离质谱（DI-EI-MS）是一种用于表征生物材料的多变量分析方法。我们证明了DI-EI-MS的使用代谢谱使用几个密切相关的地衣物种：Cladonia krempelhuberi，C。gracilis、C. pseudogymnopoda和C.多枝的该方法包括将总离子色谱图转换为积分色谱图并评估重现性。定性DI-EI-MS方法被用来配置文件中的主要和/或次要成分地衣样品提取物。通过改变DI-EI-MS中的电子能量并使用单因素方差分析检查所得数据，可以区分每个地衣样品。以前无法检测到的峰，很容易碎片可以通过改变电子能量来揭示。我们的研究结果表明，使用DI-EI-MS的代谢谱将是有用的区分亚组在同一物种。这是第一个研究报告使用的DI-EI-MS在代谢组学应用。
Direct-injection electron ionization-mass spectrometry (DI-EI-MS) is a multivariate analysis method useful for characterizing biological materials. We demonstrated the use of DI-EI-MS for metabolic profiling using several closely related lichen species: Cladonia krempelhuberi, C. gracilis, C. pseudogymnopoda, and C. ramulosa. The methodology involves conversion of total ion chromatograms to integrated chromatograms and assessment of reproducibility. The qualitative DI-EI-MS method was used to profile the major and/or minor constituents in extracts of lichen samples. It was possible to distinguish each lichen sample by altering the electron energy in DI-EI-MS and examining the resulting data using one-way analysis of variance. Previously undetectable peaks, which are easy to fragment could be revealed by varying the electron energy. Our results suggest that metabolic profiling using DI-EI-MS would be useful for discriminating between subgroups within the same species. This is the first study to report the use of DI-EI-MS in a metabolomics application.