EFFECT OF GENISTEIN ON IN-VITRO AND IN-VIVO MODELS OF CANCER
EFFECT OF GENISTEIN ON IN-VITRO AND IN-VIVO MODELS OF CANCER
复制标题
DOI：
10.1093/jn/125.3_suppl.777s
复制
发表时间：
1995-03-01
影响因子：
4.2
通讯作者：
BARNES, S
中科院分区：
文献类型：
--
作者：
BARNES, S
在关于含有染料木素的大豆原料对癌症动物模型的影响的研究中，三分之二的研究表明，癌症的风险(发病率、潜伏期或肿瘤数量)显著降低。此外，纯化的金雀异黄素延迟了乳房肿瘤的出现，与7，12-二甲基苯并[a]蒽处理的大鼠乳房组织中细胞分化的增加有关，在皮肤癌模型中抑制佛波酯诱导的过氧化氢产生，在结肠癌模型中抑制异常隐窝的形成。在体外模型中，金雀异黄素抑制体外培养的人肿瘤细胞系的增殖，其IC50值变化很大(2.6-79mU/L或1-30mU/g/mL)。只有少数病例的IC50低于13.2mU/L(5mU/g/毫升)，这是高大豆饮食人群血清染料木素浓度的假定上限。在未来的研究中，应该更加重视金雀异黄素对来自可能发生癌症的组织的未转化的正常细胞系的影响，而不是对已建立的肿瘤细胞系的影响。同样，金雀异黄素对癌症进展和/或促进的作用可能会在未转化的细胞系中更清楚地检测出来，这些细胞系被认为在肿瘤形成过程中被激活了。
In two-thirds of studies on the effect of genistein-containing soy materials in animal models of cancer, the risk of cancer (incidence, latency or tumor number) was significantly reduced. In addition, purified genistein delayed mammary tumor appearance in association with increased cell differentiation in mammary tissue in rats treated with 7,12-dimethylbenz[a]anthracene when administered neonatally, inhibited phorbol ester-induced H2O2 production in a model of skin cancer, and inhibited aberrant crypt formation in a model of colonic cancer. In in vitro models, genistein inhibited the proliferation of human tumor cell lines in culture with a wide variation in IC50 values (2.6-79 mu mol/L, or 1-30 mu g/mL). In only a few cases was the IC50 below 13.2 mu mol/L (5 mu g/mL), the presumed upper limit for the serum genistein concentration in those on a high-soy diet. In future studies, greater emphasis should be placed on the effect of genistein on nontransformed, normal cell lines from the tissues where cancer can occur rather than established tumor cell lines. Similarly, the effect of genistein on the progression and/or promotion of cancer may be more clearly examined using nontransformed cell lines transfected with specific oncogenes thought to be activated during oncogenesis.