Microbiological study and scanning electron microscopic analysis of root canal wall dentin following pumped Diodium Nd:YAG laser irradiation.
Microbiological study and scanning electron microscopic analysis of root canal wall dentin following pumped Diodium Nd:YAG laser irradiation.
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泵浦二极管 Nd:YAG 激光照射后根管壁牙本质的微生物研究和扫描电子显微镜分析。
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
R. Piccolomini
中科院分区：
文献类型：
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作者：
S. D’Ercole;C. D'Arcangelo;G. Catamo;G. D. Prosperi;D. Tripodi;G. Pasquantonio;R. Piccolomini
观察Nd：YAG激光对根管的消毒能力及激光对牙本质壁的影响。30个根管被铜绿假单胞菌ATCC 27853感染，30个根管被A. naeslundii CH-12。在每种感染中，根据治疗选择4组。其中，2个试验组（TG）分别使用2种不同的设置：1 W/70焦耳和1.5 W/100焦耳（n = 10），以15 Hz的Nd：YAG激光治疗15 s。另2组作为对照组（CG）：未处理组（阳性对照组，n = 5）和5.25%NaClO灭菌组（阴性对照组，n = 5）。扫描电镜观察及细菌计数。这些分析在感染和治疗后每组进行一次。激光治疗显著减少了这两种细菌的数量。扫描电镜调查显示，超过1.5 W/100 J的根管牙本质的熔化和结晶。激光照射具有杀菌作用，但它不完全消毒根管的NaClO 5.25%的解决方案，如果治疗的目标也是为了避免牙本质壁的改变。
The capability of Nd:YAG laser in sterilizing root canals and the alterations of dentinal walls induced by laser treatment were investigated. Thirty root canals were infected by P. aeruginosa ATCC 27853 and thirty canals by A. naeslundii CH-12. Within each infection, 4 groups were selected on the basis of the treatment. Among them, 2 test groups (TGs) were treated by Nd:YAG laser at 15 Hz for 15 s, using 2 different settings: 1 Watt/70 Joule and 1.5 Watt/100 Joule, respectively (n = 10 each). The other 2 groups, used as controls (CGs), were: untreated (positive control, n = 5) and sterilized by 5.25% NaClO group (negative control, n = 5). Observations under scanning electron microscope (SEM) and quantitative bacterial counts were performed. These analyses were performed once per group after infections and treatments. Laser treatments significantly reduced the number of both bacteria. SEM investigation showed melting and crystallization of canal dentin over 1.5 W/100 J. Laser irradiation has a bactericidal effect but it does not completely sterilize the root canal as NaClO 5.25% solution does if the goal of treatment is also to avoid alterations of dentinal walls.