The P25 pathogenicity factor of Beet necrotic yellow vein virus targets the sugar beet 26S proteasome involved in the induction of a hypersensitive resistance response via interaction with an F-box protein.
The P25 pathogenicity factor of Beet necrotic yellow vein virus targets the sugar beet 26S proteasome involved in the induction of a hypersensitive resistance response via interaction with an F-box protein.
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DOI：
10.1094/mpmi-03-12-0057-r
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发表时间：
2012-07
期刊：
影响因子：
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通讯作者：
H. Thiel;K. Hleibieh;D. Gilmer;M. Varrelmann
中科院分区：
文献类型：
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作者：
H. Thiel;K. Hleibieh;D. Gilmer;M. Varrelmann
P25是甜菜坏死黄静脉病毒（BNYVV）致病性因子，与酵母中含有未知功能F-box家族蛋白（FBK）的拟南芥（Arabidopsis thaliana kelch）重复序列高度同源的甜菜蛋白相互作用。FBK是Skp1-Cullin-F-box （SCF）复合物的成员，介导蛋白质降解。本研究证实了甜菜FBK-P25在体内和体外的相互作用，并为植物内相互作用和烟草叶细胞中类似的亚细胞分布提供了证据。P25甚至与来自a . thaliana （BNYVV非宿主）的FBK相互作用。通过证明FBK与拟南芥同源skp1样（ASK）基因（SCF E3连接酶的一个成员）的相互作用，FBK功能分类成为可能。通过酵母双杂交桥接实验，证实了P25对涉及ASK1蛋白的scf复合物形成的直接影响。农杆菌介导的FBK在拟南芥叶片中的瞬时表达诱导了超敏反应。全长F-box蛋白由一个F-box结构域和两个kelch重复序列组成，在酵母中单独不能与P25相互作用，也不会导致细胞死亡诱导。结果支持P25参与甜菜病毒致病性的观点，并提示抑制抗性反应。
P25, a Beet necrotic yellow vein virus (BNYVV) pathogenicity factor, interacts with a sugar beet protein with high homology to Arabidopsis thaliana kelch repeat containing F-box family proteins (FBK) of unknown function in yeast. FBK are members of the Skp1-Cullin-F-box (SCF) complex that mediate protein degradation. Here, we confirm this sugar beet FBK-P25 interaction in vivo and in vitro and provide evidence for in planta interaction and similar subcellular distribution in Nicotiana tabacum leaf cells. P25 even interacts with an FBK from A. thaliana, a BNYVV nonhost. FBK functional classification was possible by demonstrating the interaction with A. thaliana orthologs of Skp1-like (ASK) genes, a member of the SCF E3 ligase. By means of a yeast two-hybrid bridging assay, a direct effect of P25 on SCF-complex formation involving ASK1 protein was demonstrated. FBK transient Agrobacterium tumefaciens-mediated expression in N. benthamiana leaves induced a hypersensitive response. The full-length F-box protein consists of one F-box domain followed by two kelch repeats, which alone were unable to interact with P25 in yeast and did not lead to cell-death induction. The results support the idea that P25 is involved in virus pathogenicity in sugar beet and suggest suppression of resistance response.