Mutation analysis of Saccharomyces cerevisiae CDC6 promoter: defining its UAS domain and cell cycle regulating element.
Mutation analysis of Saccharomyces cerevisiae CDC6 promoter: defining its UAS domain and cell cycle regulating element.
复制标题
酿酒酵母CDC6启动子突变分析：定义其UAS结构域和细胞周期调控元件。
DOI：
10.1089/dna.1993.12.363
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发表时间：
1993
影响因子：
3.1
通讯作者：
Jong,AY
中科院分区：
文献类型：
--
作者：
Zhou,C;Jong,AY
Using β-galactosidase as the reporter gene, we carried out mutagenesis experiments to investigate the 5′ promoter region of theCDC6gene. Our results showed that the DNA element, between −262 and −170, is important for the upstream activating sequence (UAS) activities. On the basis of the DNA sequence, there is aMluI (−204) and aMluI-like (−216) element located within the middle of the UAS region. Insertion and deletion mutagenesis analysis of theMluI sequence has indicated that the internal CGCG sequence of theMluI site (ACGCGT) is important for gene expression. Furthermore, when DNA elements containing theMluI sites were subcloned into the tester plasmid, periodic expression of a reporter gene throughout the cell cycle was observed, as evidenced by the β-galactosidase activities andlacZmRNA. Because the possible transcriptional initiation sites of theCDC6transcript have been previously defined (Zhou and Jong, 1990, J. Biol. Chem. 264, 9022–9029), we propose a model regarding the construct of theCDC6promoter region. This 5′ promoter construct contains a UAS region and aMluI element (MCB box) typical of a family of cell cycleregulated genes involved in DNA metabolism. Previous genetic studies have not completely defined theCDC6execution point in the functional yeast cell cycle map. Our results favor the possibility that theCDC6gene is required, and directly involved, in the initiation of DNA replication.
