Relationships between free radical levels during carotid endarterectomy and markers of arteriosclerotic disease
Relationships between free radical levels during carotid endarterectomy and markers of arteriosclerotic disease
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颈动脉内膜切除术期间自由基水平与动脉硬化疾病标志物之间的关系
DOI：
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发表时间：
2007
影响因子：
3.6
通讯作者：
O. Jonsson
中科院分区：
文献类型：
--
作者：
J. Gondalia;B. Fagerberg;J. Hulthe;L. Karlström;U. Nilsson;S. Waters;O. Jonsson
背景：颈动脉内膜切除术时，从大脑流出的颈静脉血中自由基的产生量增加。本研究探讨了动脉硬化病变进展标志物、自由基产生和临床特征之间的关系。方法：使用 OXANOH 作为自旋捕获器，采用离体自旋捕获方法对 13 名患者进行了颈动脉内膜切除术期间自由基产生的研究。在颈动脉夹闭之前、期间和之后测定静脉血样中的 MCP-1、ICAM-1、MMP-9 和 oxLDL。应用主成分分析（PCA）以及偏最小二乘回归分析（PLS）来解释数据。结果：PCA 和 PLS 分析显示，MMP-9 的高值和 ICAM-1 的低值与自由基的高产生相关，而 MCP-1 和 oxLDL 与自由基的产生无关。 MMP-9 在糖尿病、高血红蛋白、高白细胞计数和血小板计数以及对侧狭窄时升高，而 ICAM-1 显示与这些临床变量的相反关系。 MCP-1 在手术期间增加。结论：我们研究的主要发现是血浆中的 MMP-9 与颈动脉内膜切除术期间自由基的产生有关，表明该酶可能参与缺血再灌注引起的脑损伤的发病机制。
Background: Free radical production is elevated in jugular venous blood emerging from the brain in conjunction with carotid endarterectomy. This study explores the relationships between markers for lesion progression in arteriosclerosis, production of radicals and clinical characteristics. Methods: The radical production during carotid endarterectomy was studied in 13 patients with an ex vivo spin trap method using OXANOH as a spin trap. MCP-1, ICAM-1, MMP-9 and oxLDL were determined in venous blood samples before, during and after clamping of the carotid artery. Principal component analysis (PCA) as well as partial least square regression analysis (PLS) was applied to interpret the data. Results: PCA and PLS analysis revealed that high values of MMP-9 and low values of ICAM-1 were associated with high radical production whereas MCP-1 and oxLDL were not correlated to radical production. MMP-9 was elevated at diabetes, high haemoglobin, high leucocyte counts and thrombocyte counts as well as at contralateral stenosis, whereas ICAM-1 showed reversed relationships to these clinical variables. MCP-1 increased during surgery. Conclusions: The main finding in our study is that MMP-9 in plasma is asscociated with radical production during carotid endarterectomy, suggesting that this enzyme might be involved in the pathogenesis of brain damage in conjunction with ischaemia-reperfusion.
影响因子：
15.9
作者：
GALIS, ZS;SUKHOVA, GK;LIBBY, P
通讯作者：
LIBBY, P