Cloning of an immunoglobulin family adhesion molecule selectively expressed by endothelial cells
Cloning of an immunoglobulin family adhesion molecule selectively expressed by endothelial cells
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DOI：
10.1074/jbc.m100630200
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发表时间：
2001-05-11
影响因子：
4.8
通讯作者：
Quertermous, T
中科院分区：
文献类型：
--
作者：
Hirata, K;Ishida, T;Quertermous, T
为了获得有关血管形成过程中内皮细胞粘附相互作用的分子基础的基本信息，我们克隆并表征了一种独特的细胞粘附分子。这种分子被命名为内皮细胞选择性粘附分子（ESAM），是免疫球蛋白超家族的新成员。由cDNA克隆编码的概念蛋白由V型和C2型免疫球蛋白结构域以及疏水信号序列、单个跨膜区和胞质结构域组成。北方印迹分析显示ESAM在培养的人和小鼠血管内皮细胞中选择性表达，并显示在肺和心脏中高水平表达，而在肾和皮肤中低水平表达。原位杂交分析表明，ESAM主要表达在胚胎的血管发育中的内皮细胞限制的模式。表位标记的ESAM显示与钙粘蛋白和连环蛋白在细胞-细胞连接处共定位。在使用表达ESAM的中国仓鼠卵巢细胞的聚集试验中，这种新型分子显示通过嗜同性相互作用介导细胞-细胞粘附。这种免疫球蛋白样粘附分子的内皮细胞选择性表达及其体外功能特征强烈表明了细胞-细胞相互作用对血管发育或功能至关重要的作用。
To gain fundamental information regarding the molecular basis of endothelial cell adhesive interactions during vascular formation, we have cloned and characterized a unique cell adhesion molecule. This molecule, named endothelial cell-selective adhesion molecule (ESAM), is a new member of the immunoglobulin superfamily. The conceptual protein encoded by cDNA clones consists of V-type and C2-type immunoglobulin domains as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. Northern blot analysis showed ESAM to be selectively expressed in cultured human and murine vascular endothelial cells and revealed high level expression in lung and heart and low level expression in kidney and skin. In situ hybridization analysis indicated that ESAM is primarily expressed in the developing vasculature of the embryo in an endothelial cell-restricted pattern. Epitope-tagged ESAM was shown to co-localize with cadherins and catenins in cell-cell junctions. In aggregation assays employing ESAM-expressing Chinese hamster ovary cells, this novel molecule was shown to mediate cell-cell adhesion through homophilic interactions. The endothelial cell-selective expression of this immunoglobulin-like adhesion molecule coupled with its in vitro functional profile strongly suggests a role in cell-cell interactions that is critical for vascular development or function.