Keratin expression in normal esophageal epithelium and squamous cell carcinoma of the esophagus.
Keratin expression in normal esophageal epithelium and squamous cell carcinoma of the esophagus.
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发表时间：
1985-02
期刊：
影响因子：
11.2
通讯作者：
Michael P. Grace;Kwan Hee Kim;Lawrence D. True;Elaine Fuchs
中科院分区：
文献类型：
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作者：
Michael P. Grace;Kwan Hee Kim;Lawrence D. True;Elaine Fuchs
8纳米角蛋白细丝是上皮细胞和上皮源性癌症(癌)细胞骨架的主要组成部分。最近，有研究表明，食道上皮细胞产生的角蛋白模式在恶变过程中发生了变化。为了评估这些差异在为病理学提供改进的诊断技术方面的潜在重要性，我们调查了正常食道上皮、食道鳞状细胞癌(SQCC)和培养的食道上皮细胞中角蛋白表达模式的一致性。在6例患者中，通过对电泳法分离的蛋白提取液进行免疫印迹分析，发现SQCC在食道和相应的正常食道上皮中表达的角蛋白模式一致不同。SCCs主要表达相对分子质量分别为58,000、56,000、50,000和46,000的角蛋白，而正常的食道上皮细胞则表达两种主要角蛋白(分子量分别为58,000和52,000)和少量角蛋白(分子量为56,000)。当正常的食道上皮细胞在组织培养中生长时，它们的角蛋白模式发生了变化，表达了相对分子质量为58,000，56,000，52,000，50,000，46,000和40,000的角蛋白。虽然角蛋白模式有一些微小的变化，但在正常食道上皮组织、食道SQCC和培养的食道细胞中表达的角蛋白模式的主要差异是一致的和可重复性的。
The 8-nm keratin filament is a major component of the cytoskeleton of epithelial cells and epithelial-derived cancers (carcinomas). Recently, it has been shown that the pattern of keratins produced by an esophageal epithelial cell undergoes change upon malignant transformation. In order to evaluate the potential importance of these differences in providing improved diagnostic techniques for pathology, we have investigated the consistency of the patterns of keratins expressed in normal esophageal epithelium, squamous cell carcinoma (SQCC) of the esophagus, and cultured esophageal epithelial cells. In six patients, the keratin pattern expressed by SQCC of the esophagus and corresponding normal esophageal epithelium was consistently different as judged by immunoblot analysis of electrophoretically separated protein extracts. Whereas the SQCCs typically expressed major keratins with molecular weights of 58,000, 56,000, 50,000, and 46,000, the normal esophageal epithelium produced two major keratins with molecular weights of 58,000 and 52,000 and a minor keratin with a molecular weight of 56,000. When normal esophageal epithelial cells were grown in tissue culture, their keratin pattern changed, and keratins with molecular weights of 58,000, 56,000, 52,000, 50,000, 46,000, and 40,000 were expressed. Although some minor variations in keratin patterns were seen, the major differences in keratin pattern expressed by normal esophageal epithelial tissue, SQCC of the esophagus, and cultured esophageal cells were consistent and reproducible.