Stability studies of Calvatia cyathiformis basidiospore allergens.
Stability studies of Calvatia cyathiformis basidiospore allergens.
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Calvatia cyathiformis 担孢子过敏原的稳定性研究。
DOI：
10.1159/000235020
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Lehrer,SB
中科院分区：
文献类型：
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作者：
Horner,WE;Ibanez,MD;Lehrer,SB
海藻变应原粗提物(粗提物)、凝胶过滤(GF)和疏水作用层析(HIC)依次分级的提取物(粗提物、凝胶过滤(GF)和疏水作用层析(HIC))中的变应原在即刻(0h)或在4、24或37℃孵育后于8、24和96h取材。Bds3.6~4.6在37℃时稳定，24℃时Bds3.6~4.6稳定96h，Bd6.6持续24 h，Bd9.3在8 h内减弱，4℃时3种变应原均稳定8、24 h，96 h Bd9.3降低，除Bds3.6~4.6外，其余变应原在低pH条件下均不稳定。蛋白酶K对Bd9.3的降解速度快于Bd6.6。免疫印迹图案与染色的凝胶相对应，并且在不同来源之间是一致的。BD9.3非常不稳定，但与63%的检测血清呈反应。从10%-15%的OFC开始。硫氰菊酯反应器只与Bd9.3结合IgE，这具有显著的变异性，对诊断和治疗具有重要意义。
Calvatia cyathiformisallergens in unfractionated extract (crude), and in extract sequentially fractionated by gel filtration (GF) and hydrophobic interaction chromatography (HIC) were tested for stability.C. cyathiformisallergen sources (crude, GF, HIC) were sampled immediately (0 h), or incubated at 4, 24 or 37 °C and then sampled at 8, 24 or 96 h. Polyacrylamide gel-isoelectric focusing immunoprints revealed 3 allergen(s) groups, or bands (Bds) with respective pi of 3.6–4.6, 6.6 and 9.3. Only Bds 3.6–4.6 were stable at 37 °C. At 24°C, Bds3.6–4.6 persisted to 96 h, Bd6.6 persisted 24 h, and Bd9.3 waned in 8 h. At 4°C all 3 allergens in HIC were stable for 8 and 24 h; Bd9.3 was reduced at 96 h. All allergen activity was labile to low pH conditions except for Bds3.6–4.6. Proteinase K degraded Bd9.3 more rapidly than Bd6.6. Immunoprint patterns corresponded to the stained gels and were consistent among different sources. Bd9.3 is very labile, but reactive with 63% of sera tested. Since 10–15% ofC. cyathiformisreactors bind IgE only to Bd9.3, this is notable variability, and significant for diagnosis and treatment.